MPST Human

Mercaptopyruvate Sulfurtransferase Human Recombinant
Cat. No.
BT10563
Source
Escherichia Coli.
Synonyms
3-mercaptopyruvate sulfurtransferase, MST, MPST, TST2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MPST Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-297) and having a molecular mass of 35kDa.
MPST is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Mercaptopyruvate sulfurtransferase (MPST) is an enzyme that facilitates the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. This enzyme plays a potential role in cysteine degradation and the detoxification of cyanide. Notably, MPST activity is regulated by oxidative stress and the protein thioredoxin. Under oxidative stress, the catalytic cysteine site of MPST is converted to a sulfenate, leading to enzyme inhibition. Conversely, reduced thioredoxin activates MPST by cleaving an intersubunit disulfide bond, effectively acting as a redox switch. Deficiencies in MPST activity have been linked to a rare inherited disorder known as mercaptolactate-cysteine disulfiduria (MCDU).
Description
Recombinant human MPST, produced in E. coli, is available as a single, non-glycosylated polypeptide chain. This protein consists of 321 amino acids (residues 1-297) and has a molecular mass of 35 kDa. The recombinant MPST is fused to a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
The MPST solution is provided at a concentration of 0.5 mg/ml and contains 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the MPST solution should be kept at 4°C. For long-term storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for extended storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of the MPST protein is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
3-mercaptopyruvate sulfurtransferase, MST, MPST, TST2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMASPQL CRALVSAQWV AEALRAPRAG QPLQLLDASW YLPKLGRDAR REFEERHIPG AAFFDIDQCS DRTSPYDHML PGAEHFAEYA GRLGVGAATH VVIYDASDQG LYSAPRVWWM FRAFGHHAVS LLDGGLRHWL RQNLPLSSGK SQPAPAEFRA QLDPAFIKTY EDIKENLESR RFQVVDSRAT GRFRGTEPEP RDGIEPGHIP GTVNIPFTDF LSQEGLEKSP EEIRHLFQEK KVDLSKPLVA TCGSGVTACH VALGAYLCGK PDVPIYDGSW VEWYMRARPE DVISEGRGKT H.

Product Science Overview

Structure and Function

MPST is a cytoplasmic enzyme that consists of 317 amino acid residues and weighs approximately 35,250 Da . It contains two rhodanese domains with similar secondary structures, suggesting a common evolutionary origin. The catalytic cysteine residue is located in the C-terminal rhodanese domain . MPST can function as a monomer or as a disulfide-linked homodimer .

The primary function of MPST is to catalyze the transfer of sulfur from 3-mercaptopyruvate to various acceptor molecules, including cyanide, which is detoxified in the process . This enzyme is also involved in the biosynthesis of thiosulfate and the production of hydrogen sulfide (H₂S), a signaling molecule with various physiological roles .

Biological Significance

MPST is widely distributed in the cytosol of mammalian cells and is conserved from bacteria to mammals . It plays a significant role in the detoxification of cyanide, a potent inhibitor of cellular respiration . Additionally, MPST is involved in the degradation of cysteine and the production of H₂S, which has been implicated in various biological processes, including vasodilation, neurotransmission, and anti-inflammatory responses .

Research and Applications

Recent studies have highlighted the importance of MPST in cancer biology. For instance, MPST has been shown to regulate cellular proliferation, migration, and bioenergetics in murine colon cancer cells . Pharmacological inhibition of MPST has been found to suppress H₂S production and attenuate cancer cell proliferation and migration . These findings suggest that MPST could be a potential therapeutic target for cancer treatment.

Human Recombinant MPST

Human recombinant MPST is a form of the enzyme that is produced using recombinant DNA technology. This allows for the production of large quantities of the enzyme for research and therapeutic purposes. Recombinant MPST is used in various biochemical assays to study its function and regulation, as well as in drug development to identify potential inhibitors that could be used in cancer therapy .

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