MORC3 Human

The MORC (Microrchidia) family CW-type zinc finger 3, commonly referred to as MORC3, is a protein encoded by the MORC3 gene in humans. This protein is part of the MORC family, which is characterized by the presence of CW-type zinc finger domains. These domains are known for their role in DNA binding and protein-protein interactions.

The MORC3 gene is located on chromosome 21 and encodes a protein that localizes to the nuclear matrix . The protein has several key structural features:

• Coiled-Coil Domains: These domains facilitate protein-protein interactions and are crucial for the formation of higher-order protein structures.
• Zinc Finger Domains: These domains are involved in binding to DNA and RNA, playing a significant role in gene regulation and expression .

MORC3 is involved in various cellular processes, including:

• Nuclear Body Formation: MORC3 forms nuclear bodies via an ATP-dependent mechanism. These nuclear bodies are involved in the regulation of gene expression and the maintenance of genomic integrity .
• RNA Binding: The protein has RNA binding activity, which suggests a role in post-transcriptional regulation of gene expression .
• Histone Methylation Reader: MORC3 can bind to different methylation states of histone H3 at lysine 4 (H3K4), indicating its involvement in chromatin remodeling and gene expression regulation .

MORC3 is associated with several biological pathways, including:

• Innate Immunity: MORC3 plays a role in the innate immune response by modulating the interferon (IFN) response. It restricts different viruses through this mechanism .
• Protein Ubiquitination: The protein is involved in the ubiquitination process, which is essential for protein degradation and regulation .

Mutations or dysregulation of the MORC3 gene have been associated with various diseases, including:

• Epidermolysis Bullosa Simplex Generalized Type: A genetic skin disorder characterized by blistering of the skin .
• Acute Sphenoidal Sinusitis: An inflammation of the sphenoid sinuses .

Recombinant MORC3 is produced using advanced biotechnological methods. It is typically expressed in systems such as SF9 insect cells and purified using chromatographic techniques. The recombinant protein is used in various research applications to study its function and role in different biological processes .