MOB3B Human

MOB Kinase Activator 3B Human Recombinant
Cat. No.
BT11527
Source
Escherichia Coli.
Synonyms
MOB Kinase Activator 3B, Chromosome 9 Open Reading Frame 35, Monopolar Spindle 1 Binding, MOB1, Domain Containing, MOB1 Mps One Binder Kinase Activator-Like 2B, Mps One Binder Kinase Activator-Like 2B, Monopolar Spindle 1 Binding, Mob1 Homolog 2b, MOBKL2B, Domain Containing, C9orf35, MOB1D, MOB1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

MOB3B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-216) and having a molecular mass of 27.9 kDa. MOB3B is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
MOB3B shares similarities with the yeast protein Mob1, known for its binding to Mps1p. Mps1p is a protein kinase crucial for spindle pole body duplication and the regulation of mitotic checkpoints. The gene encoding MOB3B is located on the opposite strand as the gene encoding the INF kappa precursor (IFNK).
Description
Recombinant human MOB3B, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 239 amino acids (residues 1-216). It has a molecular weight of 27.9 kDa. A 23 amino acid His-tag is fused to the N-terminus of MOB3B.
Physical Appearance
A clear solution that has been sterilized by filtration.
Formulation
The MOB3B solution (0.5 mg/ml) is formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 1 mM DTT, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), the solution can be stored at 4°C. For extended storage, it should be kept frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
MOB Kinase Activator 3B, Chromosome 9 Open Reading Frame 35, Monopolar Spindle 1 Binding, MOB1, Domain Containing, MOB1 Mps One Binder Kinase Activator-Like 2B, Mps One Binder Kinase Activator-Like 2B, Monopolar Spindle 1 Binding, Mob1 Homolog 2b, MOBKL2B, Domain Containing, C9orf35, MOB1D, MOB1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSIALKQ VFNKDKTFRP KRKFEPGTQR FELHKRAQAS LNSGVDLKAA VQLPSGEDQN DWVAVHVVDF FNRINLIYGT ICEFCTERTC PVMSGGPKYE YRWQDDLKYK KPTALPAPQY MNLLMDWIEV QINNEEIFPT CVGVPFPKNF LQICKKILCR LFRVFVHVYI HHFDRVIVMG AEAHVNTCYK HFYYFVTEMN LIDRKELEPL KEMTSRMCH

Product Science Overview

Introduction

MOB Kinase Activator 3B, also known as MOB3B, is a protein encoded by the MOB3B gene in humans. This protein is part of the MOB family, which plays a crucial role in various cellular processes, including cell proliferation, apoptosis, and organ size control. The MOB3B protein is particularly significant in the Hippo signaling pathway, which is essential for tumor suppression and organ size regulation.

Gene and Protein Structure

The MOB3B gene is located on chromosome 9 and is also known by several aliases, including C9orf35, FLJ13204, and MOBKL2B . The protein encoded by this gene shares similarity with the yeast Mob1 protein, which binds to Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation . The MOB3B protein modulates the expression of LATS1, a core component of the Hippo signaling pathway .

Function and Significance

MOB3B is a protein-coding gene that plays a pivotal role in the Hippo signaling pathway. This pathway is crucial for controlling organ size and suppressing tumors by restricting cell proliferation and promoting apoptosis . The MOB3B protein interacts with NDR serine-threonine kinases, which are core components of the Hippo and Hippo-like signaling pathways . These interactions are essential for the proper functioning of these pathways, making MOB3B a critical regulator of cellular processes.

Expression and Localization

The expression of MOB3B is observed in various tissues, including lymphoid tissue, bone marrow, testis, and skeletal muscle . It is involved in several biological processes, such as cell proliferation, innate immune response, protein ubiquitination, and lymph vessel development . The protein is also expressed in immune cells like NK-cells and T-cells, where it plays a role in the immune system and transcription .

Clinical Relevance

Diseases associated with MOB3B include Frontotemporal Dementia and/or Amyotrophic Lateral Sclerosis 7 . The protein’s role in the Hippo signaling pathway and its interactions with other proteins make it a potential target for therapeutic interventions in cancer and other diseases related to cell proliferation and apoptosis.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.