MOB1B Human

MOB Kinase Activator 1B Human Recombinant
Cat. No.
BT11443
Source
E.coli.
Synonyms
MOB Kinase Activator 1B, Mps One Binder Kinase Activator-Like 1A, Mob1 Homolog 1A, MOBKL1A, Mob1A, MOB4A, MOB1, Mps One Binder Kinase Activator-Like 1A (Yeast), MOB1, Mps One Binder Kinase Activator-Like 1A, MOB1 Mps One Binder Homolog B (Yeast), MOB1 Mps One Binder Homolog B, Mob4A Protein, MATS2, Mob1B, MOB kinase activator 1B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
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Description

MOB1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 239 amino acids (1-216 a.a) and having a molecular mass of 27kDa.
MOB1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MOB Kinase Activator 1B, also known as MOB1B, shares similarities with the yeast protein Mob1. In yeast, Mob1 interacts with Mps1p, a protein kinase essential for spindle pole body duplication and the regulation of the mitotic checkpoint.
Description
Recombinant human MOB1B, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 239 amino acids, with amino acids 1 to 216 representing the MOB1B sequence, and has a molecular weight of 27 kDa. A 23-amino acid His-tag is fused to the N-terminus of MOB1B. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
MOB1B is supplied as a 1 mg/ml protein solution in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 30% glycerol, and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 95% by SDS-PAGE analysis.
Synonyms
MOB Kinase Activator 1B, Mps One Binder Kinase Activator-Like 1A, Mob1 Homolog 1A, MOBKL1A, Mob1A, MOB4A, MOB1, Mps One Binder Kinase Activator-Like 1A (Yeast), MOB1, Mps One Binder Kinase Activator-Like 1A, MOB1 Mps One Binder Homolog B (Yeast), MOB1 Mps One Binder Homolog B, Mob4A Protein, MATS2, Mob1B, MOB kinase activator 1B.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSFLFGS RSSKTFKPKK NIPEGSHQYE LLKHAEATLG SGNLRMAVML PEGEDLNEWV AVNTVDFFNQ INMLYGTITD FCTEESCPVM SAGPKYEYHW ADGTNIKKPI KCSAPKYIDY LMTWVQDQLD DETLFPSKIG VPFPKNFMSV AKTILKRLFR VYAHIYHQHF DPVIQLQEEA HLNTSFKHFI FFVQEFNLID RRELAPLQEL IEKLTSKDR.

Product Science Overview

Introduction

MOB Kinase Activator 1B (MOB1B) is a protein encoded by the MOB1B gene in humans. This protein is a crucial component of the Hippo signaling pathway, which plays a significant role in regulating organ size, cell proliferation, and apoptosis (programmed cell death). The Hippo pathway is essential for maintaining tissue homeostasis and preventing tumor formation.

Gene and Protein Structure

The MOB1B gene is located on chromosome 4 and is known to produce multiple transcript variants encoding different isoforms of the protein . The protein encoded by this gene is similar to the yeast Mob1 protein, which binds to Mps1p, a protein kinase essential for spindle pole body duplication and mitotic checkpoint regulation .

Function and Mechanism

MOB1B functions as an activator of LATS1/2 kinases in the Hippo signaling pathway . The core of this pathway involves a kinase cascade where STK3/MST2 and STK4/MST1, in complex with their regulatory protein SAV1, phosphorylate and activate LATS1/2 in complex with MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ . Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus, thereby regulating genes involved in cell proliferation, cell death, and cell migration .

Biological Significance

The Hippo signaling pathway, with MOB1B as a key component, is pivotal in controlling organ size and tumor suppression by restricting cell proliferation and promoting apoptosis . Dysregulation of this pathway can lead to uncontrolled cell growth and cancer. MOB1B’s role in this pathway underscores its importance in maintaining cellular and tissue homeostasis.

Clinical Relevance

Mutations or alterations in the MOB1B gene have been associated with various diseases, including Warburg Micro Syndrome 1 . This syndrome is characterized by developmental abnormalities, including microcephaly, intellectual disability, and eye abnormalities. Understanding the function and regulation of MOB1B can provide insights into the mechanisms underlying these conditions and potentially lead to therapeutic interventions.

Expression and Localization

MOB1B is ubiquitously expressed in various tissues, with cytoplasmic localization . It is also found in the nucleoplasm and nucleoli, indicating its involvement in multiple cellular processes . The protein interacts with several other proteins, further highlighting its role in complex cellular networks .

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