MNDA Human

Myeloid Cell Nuclear Differentiation Antigen Human Recombinant
Cat. No.
BT24846
Source
Escherichia Coli.
Synonyms
PYHIN3, Myeloid cell nuclear differentiation antigen, MNDA.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MNDA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 427 amino acids (1-407 a.a.) and having a molecular mass of 47.9 kDa. The MNDA is fused to a 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
MNDA functions as a transcriptional activator and repressor in myeloid lineage and granulocyte/monocyte cell-specific responses. It enhances the DNA binding of the transcriptional repressor protein YY1. MNDA is exclusively found within the nuclei of granulocyte-monocyte lineage cells.
Description
Recombinant human MNDA, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 427 amino acids (residues 1-407) with a molecular weight of 47.9 kDa. A 20 amino acid His Tag is fused to the N-terminus of the MNDA protein. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
The MNDA solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like 0.1% HSA or BSA is advisable for long-term storage. Repeated freezing and thawing cycles should be avoided.
Purity
Purity is determined to be greater than 90% through SDS-PAGE analysis.
Synonyms
PYHIN3, Myeloid cell nuclear differentiation antigen, MNDA.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MVNEYKKILL LKGFELMDDY HFTSIKSLLA YDLGLTTKMQ EEYNRIKITD LMEKKFQGVA CLDKLIELAK DMPSLKNLVN NLRKEKSKVA KKIKTQEKAP VKKINQEEVG LAAPAPTARN KLTSEARGRI PVAQKRKTPN KEKTEAKRNK VSQEQSKPPG PSGASTSAAV DHPPLPQTSS STPSNTSFTP NQETQAQRQV DARRNVPQND PVTVVVLKAT APFKYESPEN GKSTMFHATV ASKTQYFHVK VFDINLKEKF VRKKVITISD YSECKGVMEI KEASSVSDFN QNFEVPNRII EIANKTPKIS QLYKQASGTM VYGLFMLQKK SVHKKNTIYE IQDNTGSMDV VGSGKWHNIK CEKGDKLRLF CLQLRTVDRK LKLVCGSHSF IKVIKAKKNK EGPMNVN.

Product Science Overview

Introduction

The Myeloid Cell Nuclear Differentiation Antigen (MNDA) is a protein encoded by the MNDA gene in humans. This protein is predominantly expressed in the nuclei of cells belonging to the granulocyte-monocyte lineage, which includes granulocytes and monocytes . MNDA plays a crucial role in the immune response and is involved in the regulation of transcription in myeloid cells .

Gene and Protein Structure

The MNDA gene is located on chromosome 1 at the 1q23.1 position . The gene encodes a protein consisting of 406 amino acids . MNDA is a member of the interferon-regulated 200/HIN-200 family of genes and contains a pyrin/PAAD/DAPIN domain, which is involved in apoptosis and the interferon response .

Function and Expression

MNDA is primarily expressed in myeloid cells and localizes to the nucleus . It acts as a transcriptional activator or repressor within the myeloid lineage and plays a significant role in the granulocyte/monocyte cell-specific response to interferon . The protein stimulates the DNA binding of the transcriptional repressor protein YY1 .

The expression of MNDA is upregulated in human monocytes exposed to interferon-alpha, indicating its role in the interferon response . The 1.8-kb MNDA mRNA contains an interferon-stimulated response element in the 5’ untranslated region, which is crucial for its regulation .

Clinical Significance

MNDA has been associated with various diseases, including neurodevelopmental disorders with dysmorphic facies and behavioral abnormalities, as well as herpetic gastritis . Its role in the immune response and regulation of transcription makes it a potential target for therapeutic interventions in related conditions.

Research and Applications

Recombinant MNDA protein is used in various research applications to study its function and role in the immune response. It is also utilized in the development of assays and antibodies for detecting and quantifying MNDA expression in different cell types .

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