MMP 8 Human, His

Matrix Metalloproteinase-8 Human Recombinant, His Tag
Cat. No.
BT8516
Source
Escherichia Coli.
Synonyms
CLG1, HNC, MMP-8, PMNL-CL, Neutrophil collagenase, Matrix metalloproteinase-8, MMP-8, PMNL collagenase.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MMP 8 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 390 amino acids (101-467a.a) and having a molecular mass of 44.3kDa. MMP 8 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Full-length recombinant human neutrophil pro-collagenase (MMP-8), in latent form. Matrix metalloproteinase 8 (MMP-8), also known as neutrophil collagenase, primarily degrades interstitial collagens, with a preference for collagen type I. Elevated levels of full-length MMP-8 protein have been linked to increased neutrophil infiltration in the skin, as neutrophils are the primary source of MMP-8 expression. MMP-8 is synthesized and stored within specific granules in neutrophil leukocytes. Consequently, its activity is regulated by factors like surface-bound ligands (e.g., IgG or complement components) that trigger its release via degranulation. Upon release and activation through proteolytic or oxidative mechanisms, MMP-8 plays a crucial role in the breakdown of connective tissue, a hallmark of inflammatory responses.
Description
Recombinant human MMP-8, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 390 amino acids (residues 101-467). It has a molecular weight of 44.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Sterile filtered, colorless solution.
Formulation
The MMP-8 solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 85% by SDS-PAGE analysis.
Synonyms
CLG1, HNC, MMP-8, PMNL-CL, Neutrophil collagenase, Matrix metalloproteinase-8, MMP-8, PMNL collagenase.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSLTPGNPK WERTNLTYRI RNYTPQLSEA EVERAIKDAF ELWSVASPLI FTRISQGEAD INIAFYQRDH GDNSPFDGPN GILAHAFQPG QGIGGDAHFD AEETWTNTSA NYNLFLVAAH EFGHSLGLAH SSDPGALMYP NYAFRETSNY SLPQDDIDGI QAIYGLSSNP IQPTGPSTPK PCDPSLTFDA ITTLRGEILF FKDRYFWRRH PQLQRVEMNF ISLFWPSLPT GIQAAYEDFD RDLIFLFKGN QYWALSGYDI LQGYPKDISN YGFPSSVQAI DAAVFYRSKT YFFVNDQFWR YDNQRQFMEP GYPKSISGAF PGIESKVDAV FQQEHFFHVF SGPRYYAFDL IAQRVTRVAR GNKWLNCRYG.

Product Science Overview

Introduction

Matrix Metalloproteinase-8 (MMP-8), also known as neutrophil collagenase, is a member of the matrix metalloproteinase (MMP) family. These enzymes are involved in the breakdown of extracellular matrix components, playing crucial roles in various physiological and pathological processes. MMP-8 is particularly known for its ability to degrade type I, II, and III collagens .

Gene and Protein Structure

The MMP-8 protein is encoded by the MMP8 gene, which is located on chromosome 11q22.3 in humans . The gene is part of a cluster of MMP genes. MMP-8 is synthesized as an inactive proenzyme and is stored in secondary granules within neutrophils. It is activated by autolytic cleavage .

Function and Significance

MMP-8 plays a significant role in normal physiological processes such as embryonic development, reproduction, and tissue remodeling. It is also involved in disease processes, including arthritis and metastasis . In cancer research, the loss of MMP-8 has been associated with increased tumor growth and metastatic burden, as well as enhanced tumor vascularity and altered immune cell infiltration .

Recombinant MMP-8 (Human, His Tag)

Recombinant human MMP-8 with a His tag is a form of the protein that has been genetically engineered for research purposes. The His tag is a sequence of histidine residues added to the protein to facilitate purification and detection. This recombinant protein is typically expressed in systems such as HEK 293 cells or E. coli .

  • Expression System: HEK 293 cells or E. coli
  • Purity: Greater than 90%
  • Endotoxin Level: Less than 1 EU/µg
  • Applications: Suitable for SDS-PAGE and other biochemical assays .
Applications in Research

Recombinant MMP-8 is widely used in biochemical and medical research to study its role in various physiological and pathological processes. It is particularly useful in cancer research, where it helps in understanding the mechanisms of tumor growth and metastasis .

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MMP 8 Human
Matrix Metalloproteinase-8 Human Recombinant
Cat. No.
BT8425
Source
Escherichia Coli.
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