MMP 3 Human, GST

Matrix Metalloproteinase-3 Human Recombinant, GST Tag
Cat. No.
BT8147
Source
Escherichia Coli.
Synonyms
Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.
Appearance
Sterile Filtered clear solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MMP-3 Human Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain fused to a GST tag containing 228 amino acids (251-478) and having a total molecular mass of 51kDa.
MMP-3 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Matrix metalloproteinase-3 (MMP-3), also known as stromelysin-1 or transin-1, is an enzyme that plays a significant role in the breakdown of extracellular matrix (ECM) components. This enzyme exhibits activity at physiological pH and temperature, effectively degrading various ECM components, including collagen, proteoglycans, laminin, fibronectin, and gelatin. Notably, MMP-3 demonstrates the ability to hydrolyze collagen types III, IV, and IX. Its involvement in the degradation of the intervertebral nucleus pulposus and annulus fibrosus makes it relevant to the study of herniated lumbar intervertebral discs. Furthermore, MMP-3 exhibits the capacity to activate other MMPs, such as pro-MMP-9 and pro-MMP-8, thereby amplifying its impact on ECM remodeling. It also contributes to the activation of MMP-1 by plasmin. Existing in a latent proenzyme form, MMP-3 undergoes activation through the action of various proteinases, including plasmin, trypsin, chymotrypsin, cathepsin G, and human neutrophil elastase. Research suggests a potential role for MMP-3 in inflammation, as it has been observed to activate the precursor of interleukin-1 beta (IL-1β).
Description
Recombinant human MMP-3, expressed in E. coli, is a monomeric protein. It consists of a single polypeptide chain devoid of glycosylation. The protein includes a GST tag and comprises 228 amino acids (residues 251-478), resulting in a molecular weight of 51 kDa. The purification of MMP-3 is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile-filtered.
Formulation
MMP-3 is provided in a buffer solution consisting of 50 mM Tris-acetate (pH 7.5), 1 mM EDTA, and 20% glycerol.
Stability
For optimal storage, maintain the product at 4°C if the entire vial is intended for use within 2-4 weeks. For extended storage, store the product frozen at -20°C. Repeated freeze-thaw cycles should be avoided to preserve protein integrity.
Synonyms
Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.
Source
Escherichia Coli.

Product Science Overview

Introduction

Matrix Metalloproteinase-3 (MMP-3), also known as Stromelysin-1, is an enzyme that plays a crucial role in the degradation of extracellular matrix (ECM) components. This enzyme is part of the matrix metalloproteinase (MMP) family, which is involved in various physiological and pathological processes, including tissue remodeling, wound healing, and cancer metastasis .

Structure and Function

MMP-3 is encoded by the MMP3 gene located on chromosome 11q22.3 . The enzyme has an estimated molecular weight of 54 kDa . MMP-3 can degrade multiple ECM components such as proteoglycans, fibronectin, laminin, and type IV collagen . It also activates other pro-MMPs, initiating a cascade of MMP-mediated degradation reactions .

Human Recombinant MMP-3 with GST Tag

The human recombinant form of MMP-3 is produced using recombinant DNA technology. This involves inserting the MMP3 gene into a suitable expression system, such as E. coli or HEK293 cells, to produce the enzyme in large quantities . The recombinant enzyme is often tagged with Glutathione S-transferase (GST) to facilitate purification and detection. The GST tag allows the enzyme to be purified using affinity chromatography, which is a highly efficient method for isolating recombinant proteins .

Applications

Recombinant MMP-3 is widely used in research to study its role in various biological processes and diseases. It is used to investigate the mechanisms of ECM degradation, tissue remodeling, and the activation of other MMPs. Additionally, MMP-3 is studied for its potential therapeutic applications in conditions such as arthritis, cancer, and cardiovascular diseases .

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