MMP28 Human

MMP-28 is a secreted protein that belongs to the peptidase M10A family . It is produced by proliferating keratinocytes and is involved in the degradation of casein . The enzyme is constitutively expressed by epithelial cells in many tissues, including the respiratory epithelium in the lung and keratinocytes in the skin . This constitutive expression suggests that MMP-28 plays a role in epithelial cell homeostasis .

MMP-28 is involved in the breakdown of the extracellular matrix, which is essential for normal physiological processes such as tissue remodeling, reproduction, and embryonic development . It is also implicated in disease processes like asthma and metastasis . The enzyme is known to mediate the degradation of casein and its expression is upregulated in response to skin injury, indicating its role in tissue repair and homeostasis .

Studies have shown that MMP-28 contributes to airway epithelial cell survival . Over-expression of MMP-28 provides protection against apoptosis induced by serum-deprivation or treatment with a protein kinase inhibitor . Additionally, increased caspase-3/7 activity has been observed in influenza-infected lungs from Mmp28-/- mice compared to wild-type mice, suggesting a role in promoting epithelial cell survival in the lung .

Recombinant human MMP-28 is typically produced in E. coli and is available with His tags for purification . The recombinant protein is used in research to study its function and role in various physiological and pathological processes. It is usually lyophilized from sterile PBS and can be reconstituted with sterile water to prepare a stock solution .