MMP23B Human
Matrix Metallopeptidase 23B Human Recombinant
Cat. No.
BT9369
Source
Escherichia Coli.
Synonyms
Matrix Metallopeptidase 23B, MMP23B, MMP22, Matrix Metalloproteinase 23B, Matrix Metalloproteinase 22, Matrix Metalloproteinase In The Female Reproductive Tract, Matrix Metalloproteinase-21, Matrix Metalloproteinase-22, MIFR-1, MMP-21, MMP-22, MMP-23, MIFR, MMP23A, Matrix Metalloproteinase-23, EC 3.4.24.-, Femalysin, MMP21.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MMP23B Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 199 amino acids (79-254) and having a molecular mass of 22.6kDa.
MMP23B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MMP23B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Matrix metalloproteinase 23B (MMP23B), a member of the matrix metalloproteinase (MMP) family, resides within a duplicated region on chromosome 1p36.3. This protease plays a crucial role in regulating the surface expression of certain potassium channels by retaining them within the endoplasmic reticulum. The MMP family broadly contributes to the breakdown of the extracellular matrix, participating in both normal physiological processes (e.g., embryonic development, reproduction, tissue remodeling) and disease processes (e.g., arthritis, metastasis).
Description
Recombinant human MMP23B, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 199 amino acids (residues 79-254) and exhibiting a molecular weight of 22.6 kDa. This protein is fused to a 23 amino acid His-tag at the N-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A sterile, colorless solution.
Formulation
The MMP23B solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), maintain the product at 4°C. For extended storage, freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid subjecting the product to repeated freeze-thaw cycles.
Purity
Purity exceeds 85% as determined by SDS-PAGE analysis.
Synonyms
Matrix Metallopeptidase 23B, MMP23B, MMP22, Matrix Metalloproteinase 23B, Matrix Metalloproteinase 22, Matrix Metalloproteinase In The Female Reproductive Tract, Matrix Metalloproteinase-21, Matrix Metalloproteinase-22, MIFR-1, MMP-21, MMP-22, MMP-23, MIFR, MMP23A, Matrix Metalloproteinase-23, EC 3.4.24.-, Femalysin, MMP21.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSYTLTPAR LRWDHFNLTY RILSFPRNLL SPRETRRALA AAFRMWSDVS PFSFREVAPE QPSDLRIGFY PINHTDCLVS ALHHCFDGPT GELAHAFFPP HGGIHFDDSE YWVLGPTRYS WKKGVWLTDL VHVAAHEIGH ALGLMHSQHG RALMHLNATL RGWKALSQDE LWGLHRLYG.
Product Science Overview
Gene and Protein Structure
The MMP23B gene is located on chromosome 1p36.3 and is part of a duplicated region. This gene encodes a protein that is involved in the regulation of ECM degradation. The protein structure of MMP23B includes a signal peptide, a propeptide, a catalytic domain, and a hemopexin-like domain. The catalytic domain contains a zinc-binding motif, which is essential for its enzymatic activity .
Function and Mechanism
MMP23B is a protease that may regulate the surface expression of certain potassium channels by retaining them in the endoplasmic reticulum. This regulation is crucial for maintaining cellular homeostasis and function. The enzyme’s activity is tightly controlled by tissue inhibitors of metalloproteinases (TIMPs), which bind to the active site of MMPs and inhibit their proteolytic activity .
Physiological and Pathological Roles
MMP23B is involved in several physiological processes, including:
- Embryonic Development: MMP23B plays a role in the remodeling of the ECM, which is essential for tissue and organ development during embryogenesis.
- Reproduction: The enzyme is involved in the breakdown of ECM components in the female reproductive tract, facilitating processes such as ovulation and implantation.
- Tissue Remodeling: MMP23B contributes to the remodeling of tissues during wound healing and other regenerative processes .
In pathological conditions, dysregulation of MMP23B activity can lead to various diseases, including:
Clinical Significance
Research and Therapeutic Applications
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