MMP1 (24-207) Human
HEK293 Cells.
nterstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN.
Greater than 95.0% as determined by SDS-PAGE.
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Description
MMP1 (24-207) Human Recombinant is a single, glycosylated polypeptide chain containing 190 amino acids and having a molecular mass of 21.2kDa. MMP1 (24-207) is fused to a 6 a.a C-terminal His tag.
Product Specs
nterstitial collagenase, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, MMP1, CLG, CLGN.
HEK293 Cells.
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIAHHHHHH.
Product Science Overview
MMP-1 is a zinc-dependent endopeptidase that can degrade a wide range of substrates, including types I, II, III, VII, VIII, and X collagens, as well as other ECM proteins like L-Selectin, pro-TNF, IGFBP-3, IGFBP-5, casein, gelatin, and myelin basic protein . The enzyme is synthesized as an inactive proenzyme and requires activation to become functional. The active form of MMP-1 contains a catalytic domain with a zinc-binding site, which is essential for its enzymatic activity .
The recombinant form of MMP-1 (24-207 a.a) is a truncated version of the full-length protein, containing amino acids 24 to 207. This version is expressed in HEK293 cells, a human embryonic kidney cell line, which ensures proper post-translational modifications such as glycosylation . The recombinant protein is a single, glycosylated polypeptide chain with a molecular mass of approximately 21.2 kDa and is fused to a 6 a.a C-terminal His tag for purification purposes .
Recombinant MMP-1 (24-207 a.a) is widely used in biochemical research to study the enzyme’s role in ECM degradation and its involvement in various pathological conditions, including cancer, arthritis, and cardiovascular diseases. The enzyme’s ability to cleave fibrillar collagens makes it a valuable tool for investigating tissue remodeling processes .
The lyophilized form of recombinant MMP-1 (24-207 a.a) should be stored at -20°C to maintain its stability. Upon reconstitution, the protein can be stored at 4°C for a limited period, typically up to two weeks, without significant loss of activity. It is recommended to aliquot the reconstituted protein to avoid repeated freeze-thaw cycles .
