MMP14 Human, His

Matrix Metalloproteinase-14 Human Recombinant, His Tag
Cat. No.
BT9004
Source
Sf9, Baculovirus cells.
Synonyms
Matrix Metallopeptidase 14, Matrix Metallopeptidase 14 (Membrane-Inserted), Membrane-Type-1 Matrix Metalloproteinase, Membrane Type 1 Metalloprotease, EC 3.4.24.80, MT-MMP 1, MT1-MMP, MMP-14, MMP-X1, MT1MMP, MTMMP1, Matrix Metalloproteinase 14 (Membrane-Inserted), Membrane-Type Matrix Metalloproteinase 1, Matrix Metalloproteinase-14, EC 3.4.24, MT-MMP, WNCHRS, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

MMP14 Human Recombinant produced in Sf9 Baculovirus cells is a single, non-glycosylated polypeptide chain containing 527 amino acids (21-538a.a) and having a molecular mass of 59.9kDa (Molecular size on SDS-PAGE will appear at approximately 35-70kDa). 
MMP14 is fused to a 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Matrix metalloproteinase-14 (MMP14), also known as MT1-MMP, is a membrane-bound enzyme that plays a crucial role in the breakdown and remodeling of the extracellular matrix (ECM). It is frequently found at the forefront of invasive tumors, contributing to tumor cell invasion by degrading the ECM. MMP14 exhibits the ability to degrade type I collagen, activate pro-MMP-2, and process cell adhesion molecules such as CD44 and integrin alpha V. This enzyme is essential for various physiological and pathological processes, including angiogenesis and tumor invasion.
Description
Recombinant human MMP14, expressed in Sf9 insect cells using a baculovirus expression system, is a single, non-glycosylated polypeptide chain. This protein consists of 527 amino acids (21-538a.a), with a molecular weight of 59.9 kDa. Note that the apparent molecular size on SDS-PAGE may vary, appearing between 35-70 kDa. The MMP14 protein has a 6 amino acid His-tag fused at its C-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The MMP14 protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer containing Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the MMP14 protein should be stored at 4°C. For long-term storage, it is recommended to store the protein at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as HSA or BSA to a final concentration of 0.1%. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of MMP14 is greater than 90% as determined by SDS-PAGE analysis.
Synonyms
Matrix Metallopeptidase 14, Matrix Metallopeptidase 14 (Membrane-Inserted), Membrane-Type-1 Matrix Metalloproteinase, Membrane Type 1 Metalloprotease, EC 3.4.24.80, MT-MMP 1, MT1-MMP, MMP-14, MMP-X1, MT1MMP, MTMMP1, Matrix Metalloproteinase 14 (Membrane-Inserted), Membrane-Type Matrix Metalloproteinase 1, Matrix Metalloproteinase-14, EC 3.4.24, MT-MMP, WNCHRS, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADLALASLGS AQSSSFSPEA WLQQYGYLPP GDLRTHTQRS PQSLSAAIAA MQKFYGLQVT GKADADTMKA MRRPRCGVPD KFGAEIKANV RRKRYAIQGL KWQHNEITFC IQNYTPKVGE YATYEAIRKA FRVWESATPL RFREVPYAYI REGHEKQADI MIFFAEGFHG DSTPFDGEGG FLAHAYFPGP NIGGDTHFDS AEPWTVRNED LNGNDIFLVA VHELGHALGL EHSSDPSAIM APFYQWMDTE NFVLPDDDRR GIQQLYGGES GFPTKMPPQP RTTSRPSVPD KPKNPTYGPN ICDGNFDTVA MLRGEMFVFK ERWFWRVRNN QVMDGYPMPI GQFWRGLPAS INTAYERKDG KFVFFKGDKH WVFDEASLEP GYPKHIKELG RGLPTDKIDA ALFWMPNGKT YFFRGNKYYR FNEELRAVDS EYPKNIKVWE GIPESPRGSF MGSDEVFTYF YKGNKYWKFN NQKLKVEPGY PKSALRDWMG CPSGGRPDEG TEEETEVIII EVDEEGGGAV SHHHHHH.

Product Science Overview

Introduction

Matrix Metalloproteinase-14 (MMP-14), also known as MT1-MMP (Membrane-Type 1 Matrix Metalloproteinase), is a member of the matrix metalloproteinase (MMP) family. These enzymes are involved in the breakdown of extracellular matrix (ECM) components, playing crucial roles in various physiological and pathological processes, including tissue remodeling, embryonic development, and disease progression such as cancer metastasis .

Structure and Function

MMP-14 is a membrane-anchored zinc-binding endopeptidase. Unlike most MMPs, which are secreted as inactive proenzymes and activated extracellularly, MMP-14 is tethered to the cell surface via a transmembrane domain . This localization is essential for its function in pericellular proteolysis, where it degrades ECM components such as collagen .

One of the key roles of MMP-14 is the activation of progelatinase A (MMP-2), which further contributes to ECM degradation. This activity is particularly important in processes like tumor invasion, where MMP-14 facilitates the breakdown of ECM barriers, allowing cancer cells to invade surrounding tissues .

Recombinant MMP-14 (Human, His Tag)

Recombinant MMP-14 (Human, His Tag) is a form of the enzyme produced through recombinant DNA technology. This involves inserting the gene encoding MMP-14 into a host organism, such as bacteria or yeast, which then expresses the protein. The “His Tag” refers to a sequence of histidine residues added to the protein to facilitate purification using affinity chromatography .

Applications

Recombinant MMP-14 is widely used in research to study its role in ECM remodeling and its involvement in various diseases. It is also used in drug discovery to screen for inhibitors that could potentially be used as therapeutic agents in conditions like cancer and fibrosis .

Clinical Significance

MMP-14 has been implicated in several pathological conditions. Overexpression of MMP-14 is often observed in various cancers and is associated with increased tumor invasiveness and poor prognosis. Additionally, deficits in MMP-14 activity have been linked to conditions such as Winchester syndrome and multicentric osteolysis-nodulosis-arthropathy spectrum .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

MMP14 Human
Matrix Metalloproteinase-14 Recombinant Human
Cat. No.
BT8950
Source
<p><span lang="EN-US">Escherichia Coli.</span></p>
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.