MMP 13 Human
CLG3, MANDP1, Matrix metalloproteinase-13, MMP-13, MMP13.
Greater than 85% as determined by SDS-PAGE.
Description
Product Specs
Matrix metalloproteinase-13 (MMP-13) is an enzyme belonging to the MMP extracellular protease family. These proteases have broad substrate specificities and contribute to both normal and diseased tissue proliferation. MMP-13 targets various molecules, including collagen, gelatin, entactin, pro-TNF-alpha, and chemokine SDF-11-4. Initially, MMP-13 exists in its inactive form as a glycosylated proenzyme with a molecular weight of 52-56 kDa. Upon cleavage, it transforms into the active MMP-13 form, weighing 22-46 kDa, which plays a crucial role in extracellular matrix remodeling. Due to its significant involvement in cell migration and metastasis, MMP-13 is a key target for inhibition studies.
The purity of MMP-13 is determined to be greater than 85% using SDS-PAGE analysis.
CLG3, MANDP1, Matrix metalloproteinase-13, MMP-13, MMP13.
Product Science Overview
Matrix Metalloproteinase-13 (MMP-13), also known as collagenase-3, is a member of the matrix metalloproteinase (MMP) family. These enzymes are involved in the breakdown of extracellular matrix (ECM) components, which is crucial for various physiological processes such as tissue remodeling, wound healing, and embryonic development. MMP-13 is particularly known for its ability to degrade collagen, a major structural protein in the ECM.
MMP-13 is a zinc-dependent endopeptidase that is synthesized as a proenzyme (proMMP-13) and requires activation to become fully functional. The human recombinant form of MMP-13 is typically expressed in Escherichia coli (E. coli) and purified using chromatographic techniques. The recombinant protein often includes a His-tag to facilitate purification and detection .
MMP-13 plays a significant role in the degradation of various types of collagen, including type I, II, III, IV, X, and XIV. It has the highest activity with soluble type II collagen. The enzyme is involved in several physiological and pathological processes:
- Tissue Remodeling: MMP-13 is essential for the remodeling of the ECM during development and wound healing. It helps in the turnover of collagen and other matrix components.
- Bone Development: MMP-13 is required for normal embryonic bone development and ossification. It plays a role in the healing of bone fractures via endochondral ossification.
- Cartilage Degradation: MMP-13 is implicated in the degradation of cartilage in diseases such as osteoarthritis. It promotes the irreversible destruction of cartilage collagen .
Due to its role in ECM degradation, MMP-13 is a target for therapeutic intervention in various diseases. Inhibitors of MMP-13 are being explored for the treatment of conditions like osteoarthritis, where excessive collagen degradation leads to joint damage. Additionally, MMP-13 is being studied for its role in cancer metastasis, as it can facilitate tumor cell invasion by degrading the ECM .
Recombinant human MMP-13 is used extensively in research to study its structure, function, and role in various diseases. The recombinant protein is typically produced in E. coli and purified to high purity levels (>95%) for use in biochemical assays and structural studies. It is available in both proenzyme and active forms, allowing researchers to investigate the activation mechanisms and enzymatic activity of MMP-13 .
