Introduction
Matrix metalloproteinase 10 (MMP10), a member of the matrix metalloproteinase (MMP) family, plays a crucial role in activating procollagenase. MMP10 is part of a gene cluster located on chromosome 11q22.3. This enzyme is involved in the breakdown of the extracellular matrix, a process essential for normal physiological functions such as embryonic development, reproduction, and tissue remodeling. Additionally, MMP10 is implicated in disease processes like arthritis and metastasis. Most MMPs are secreted as inactive precursors (proproteins) that require cleavage by extracellular proteinases for activation. Specifically, MMP10 encodes an enzyme responsible for degrading proteoglycans and fibronectin.
Description
Recombinant human MMP10, produced in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 401 amino acids (99-476a.a), resulting in a molecular weight of 45.4 kDa. The MMP10 sequence includes a 23 amino acid His-tag fused at the N-terminus to facilitate purification, which is achieved through proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MMP10 solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.4M urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the MMP10 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain protein integrity.
Purity
Purity is determined by SDS-PAGE analysis and exceeds 90%.
Synonyms
SL-2, STMY2, Stromelysin-2, Matrix metalloproteinase-10, MMP-10, Transin-2, MMP10.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSFSSFPGM PKWRKTHLTY RIVNYTPDLP RDAVDSAIEK ALKVWEEVTP LTFSRLYEGE ADIMISFAVK EHGDFYSFDG PGHSLAHAYP PGPGLYGDIH FDDDEKWTED ASGTNLFLVA AHELGHSLGL FHSANTEALM YPLYNSFTEL AQFRLSQDDV NGIQSLYGPP PASTEEPLVP TKSVPSGSEM PAKCDPALSF DAISTLRGEY LFFKDRYFWR RSHWNPEPEF HLISAFWPSL PSYLDAAYEV NSRDTVFIFK GNEFWAIRGN EVQAGYPRGI HTLGFPPTIR KIDAAVSDKE KKKTYFFAAD KYWRFDENSQ SMEQGFPRLI ADDFPGVEPK VDAVLQAFGF FYFFSGSSQF EFDPNARMVT HILKSNSWLH C.
