MIS12 Human
Description
MIS12 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 228 amino acids (1-205 a.a.) and having a molecular mass of 26.5kDa. MIS12 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MGSSHHHHHH SSGLVPRGSH MGSMSVDPMT YEAQFFGFTP QTCMLRIYIA FQDYLFEVMQ AVEQVILKKL DGIPDCDISP VQIRKCTEKF LCFMKGHFDN LFSKMEQLFL QLILRIPSNI LLPEDKCKET PYSEEDFQHL QKEIEQLQEK YKTELCTKQA LLAELEEQKI VQAKLKQTLT FFDELHNVGR DHGTSDFRES LVSLVQNSRK LQNIRDNVEK ESKRLKIS.
Product Science Overview
MIS12 is a crucial protein component of the kinetochore, a structure essential for chromosome segregation during cell division. The kinetochore is responsible for the attachment of chromosomes to spindle microtubules, ensuring accurate chromosome alignment and segregation during mitosis. The MIS12 complex, which includes MIS12, DSN1, NSL1, and PMF-1, plays a pivotal role in this process .
The MIS12 complex is part of the KMN network, which also includes the NDC80 and KNL1 complexes. This network forms the core of the outer kinetochore, creating an interface that connects microtubules to centromeric chromatin . The MIS12 complex has an elongated structure with a long axis of approximately 22 nm . The NSL1 subunit acts as a scaffold, supporting interactions between the MIS12 complex and the NDC80 and KNL1 complexes .
Recombinant human MIS12 protein is produced in E. coli and is fused to a His-tag at the N-terminus. This recombinant protein corresponds to the amino acids 1-205 of human MIS12 and has a molecular mass of approximately 26.5 kDa . The recombinant protein is typically purified using proprietary chromatographic techniques to achieve a purity of over 90% .
Recombinant human MIS12 is primarily used in research to study the molecular mechanisms of kinetochore function and chromosome segregation. It is also used to investigate the interactions between different components of the KMN network and to understand the structural organization of the kinetochore .
