MIP-1 Gamma Mouse

Macrophage Inflammatory Protein-1 Gamma (MIP-1γ), also known as CCL9, is a member of the CC chemokine family. This protein is primarily found in murine (mouse) blood and various tissues, with no known human homolog . MIP-1γ plays a crucial role in the immune response by attracting neutrophils and inhibiting the colony formation of bone marrow myeloid immature progenitors .

MIP-1γ is a small cytokine that belongs to the CC chemokine family. It is encoded by the CCL9 gene located on mouse chromosome 11 . The protein is expressed constitutively, mainly in macrophages and bone marrow cells . The recombinant form of MIP-1γ is produced using Escherichia coli expression systems and is highly pure, with a purity greater than 98% as determined by SDS-PAGE and HPLC analyses .

MIP-1γ is known for its inflammatory, pyrogenic, and chemokinetic properties. It circulates at high concentrations in the blood of healthy animals . The protein binds to the CCR1 receptor, which activates calcium release in neutrophils . This binding leads to the chemoattraction of neutrophils, which are essential for the immune response . Additionally, MIP-1γ inhibits the colony formation of bone marrow myeloid immature progenitors, thereby playing a role in hematopoiesis .

Recombinant MIP-1γ is widely used in various research applications, including functional studies, SDS-PAGE, and ELISA . It serves as a positive control in Western Blot analysis and is used to study the immune response and inflammatory processes . The protein’s ability to attract neutrophils and inhibit progenitor colony formation makes it a valuable tool for studying immune cell behavior and hematopoiesis .

Recombinant MIP-1γ is typically shipped at 4°C and should be stored at +4°C for short-term use (1-2 weeks). For long-term storage, it should be aliquoted and kept at -20°C or -80°C to avoid freeze/thaw cycles . The protein is lyophilized and should be reconstituted with sterile water to a concentration of 0.1 mg/ml before use .