MICA Human, His

MHC class I chain-related gene A, Human Recombinant His Tag

Cat. No.

BT11913

Source

Escherichia Coli.

Synonyms

MHC class I polypeptide-related sequence A, MIC-A, MICA, PERB11.1, HLA-B, AS, HLAB, HLAC, SPDA1, HLA-B73, HLA-B-7301.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MICA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (24-297aa) and having a molecular mass of 32.7kDa.
MICA is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that acts as a ligand for the human NKG2D receptor. MICB, a similar protein, shares 85% amino acid identity with MICA. These proteins are distantly related to MHC class I proteins. They possess three extracellular Ig-like domains but lack the ability to bind peptides or interact with β2-microglobulin. The genes encoding these proteins are located within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic, with over 50 recognized human alleles. While MICA is absent from most cells, it is often expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA serves as a ligand for human NKG2D, an activating receptor found on NK cells, NKT cells, γδ T cells, and CD8+ β T cells. Recognition of MICA by NKG2D triggers the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition plays a role in tumor surveillance, viral infections, and autoimmune diseases.

Description

Recombinant human MICA, produced in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 283 amino acids (24-297aa) and has a molecular mass of 32.7 kDa. The protein includes an 8 amino acid His-tag fused at the C-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The MICA protein solution (0.25 mg/ml) is supplied in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.

Stability

For short-term storage (up to 4 weeks), store the vial at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. It is crucial to avoid repeated freeze-thaw cycles.

Purity

The purity is greater than 90.0% as determined by SDS-PAGE analysis.

Synonyms

MHC class I polypeptide-related sequence A, MIC-A, MICA, PERB11.1, HLA-B, AS, HLAB, HLAC, SPDA1, HLA-B73, HLA-B-7301.

Source

Escherichia Coli.

Amino Acid Sequence

MEPHSLRYNL TVLSWDGSVQ SGFLTEVHLD GQPFLRCDRQ KCRAKPQGQW AEDVLGNKTW DRETRDLTGN GKDLRMTLAH IKDQKEGLHS LQEIRVCEIH EDNSTRSSQH FYYDGELFLS QNLETEEWTM PQSSRAQTLA MNVRNFLKED AMKTKTHYHA MHADCLQELR RYLKSGVVLR RTVPPMVNVT RSEASEGNIT VTCRASGFYP WNITLSWRQD GVSLSHDTQQ WGDVLPDGNG TYQTWVATRI CQGEEQRFTC YMEHSGNHST HPVPSLEHHH HHH.

Product Science Overview

Introduction

The MHC class I chain-related gene A (MICA) is a transmembrane glycoprotein that plays a crucial role in the immune system. It functions as a ligand for the human Natural-Killer Group 2 Member D (NKG2D) receptor, which is involved in the activation of natural killer (NK) cells and some T cells . MICA is part of the non-classical MHC class I family, which also includes MICB, a closely related protein sharing a high degree of sequence identity .

Structure and Expression

MICA is a single-pass type I membrane protein expressed on the cell surface of various cell types, including gastric epithelium, endothelial cells, fibroblasts, keratinocytes, and monocytes. Its expression can be induced by stress conditions such as bacterial and viral infections. The protein consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain is responsible for binding to the NKG2D receptor.

Recombinant MICA with His Tag

Recombinant MICA proteins are often produced with a His tag, a sequence of histidine residues added to the protein to facilitate purification and detection. The His tag allows for easy purification using nickel-affinity chromatography and can be detected using anti-His antibodies. Recombinant MICA with a His tag is typically produced in Chinese Hamster Ovary (CHO) cells, ensuring proper folding and post-translational modifications.

Functional Significance

MICA’s interaction with the NKG2D receptor is critical for the immune response. When MICA binds to NKG2D, it activates NK cells and some T cells, leading to the destruction of infected or transformed cells. This mechanism is essential for the body’s defense against tumors and infections. Additionally, MICA expression can be upregulated in response to cellular stress, further enhancing its role in immune surveillance.

Industrial Applications

Recombinant MICA with a His tag is used in various research and clinical applications. It is employed in studies investigating the immune response, cancer immunotherapy, and the development of diagnostic tools. The ability to produce recombinant MICA in large quantities allows for detailed studies of its structure and function, contributing to our understanding of immune regulation and potential therapeutic interventions .

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MICA Human, His

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