MGP Human
Description
The MGP is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
MGP contains five to six residues of Gla, a calcium-binding amino acid that requires vitamin K-dependent gamma-carboxylase for its formation . The protein acts as an inhibitor of vascular mineralization and is involved in bone organization . It is found in various body tissues, including bone, cartilage, heart, and kidney . In bone, MGP works alongside osteocalcin, another vitamin K-dependent protein, to participate in the organization of bone tissue .
The MGP gene is located on the short arm of chromosome 12 in humans . Its mRNA sequence is 585 bases long . Abnormalities in the MGP gene have been linked to Keutel syndrome, a rare condition characterized by abnormal calcium deposition in cartilage, peripheral stenosis of the pulmonary artery, and midfacial hypoplasia . Mice lacking MGP develop arterial calcification, leading to blood-vessel rupture and early death .
Recombinant human MGP is produced using E. coli expression systems and is often fused with a His-tag at the N-terminus for purification purposes . The recombinant protein is typically denatured using detergents during the purification process . This form of MGP is used in various research applications, including Western Blot and imaging assays .
MGP is synthesized in a vitamin K-dependent manner in smooth muscle cells of the healthy vessel wall, and its mRNA transcription is significantly upregulated in atherosclerotic lesions . Additionally, MGP has been found to have immunomodulatory functions and is associated with conditions such as ulcerative colitis .
