MGAT2 Human, Sf9
Sf9, Baculovirus cells.
Alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, MGAT2, CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2, Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II.
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
MGAT2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 427 amino acids (30-447a.a.) and having a molecular mass of 49.3kDa. (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
MGAT2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The enzyme MGAT2 plays a critical role in the conversion of oligomannose to complex N-glycans. This enzyme is composed of three distinct domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain, a typical structure found in glycosyltransferases. The MGAT2 gene in humans is responsible for encoding this enzyme. Notably, the DNA sequence coding for MGAT2 lacks introns. Consequently, any mutations within the MGAT2 gene can lead to a disorder known as carbohydrate-deficient glycoprotein syndrome, type II.
Produced in Sf9 Baculovirus cells, MGAT2 is a single, glycosylated polypeptide chain that contains 427 amino acids (specifically, amino acids 30 to 447) and has a molecular mass of 49.3 kDa. It's important to note that on SDS-PAGE, the molecular size will appear to be between 40 kDa and 57 kDa. MGAT2 is engineered with a 6 amino acid His tag at its C-Terminus and undergoes purification using specialized chromatographic techniques.
The product is a colorless solution that has been sterilized by filtration.
The MGAT2 protein solution is provided at a concentration of 0.25 mg/ml and contains 20mM Tris-HCl (pH 7.5), 10% glycerol, 0.15M NaCl, and 1mM DTT.
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To further enhance long-term stability, consider adding a carrier protein (either HSA or BSA) to a final concentration of 0.1%. It's important to avoid repeated freeze-thaw cycles to maintain product integrity.
The purity of MGAT2 is determined to be greater than 90% using SDS-PAGE analysis.
Alpha-1, 6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, MGAT2, CDG2A, CDGS2, GLCNACTII, GNT-II, GNT2, Beta-1,2-N-acetylglucosaminyltransferase II, GlcNAc-T II, Mannoside acetylglucosaminyltransferase 2, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II.
Sf9, Baculovirus cells.
ADPRQRKNEA LAPPLLDAEP ARGAGGRGGD HPSVAVGIRR VSNVSAASLV PAVPQPEADN LTLRYRSLVY QLNFDQTLRN VDKAGTWAPR ELVLVVQVHN RPEYLRLLLD SLRKAQGIDN VLVIFSHDFW STEINQLIAG VNFCPVLQVF FPFSIQLYPN EFPGSDPRDC PRDLPKNAAL
KLGCINAEYP DSFGHYREAK FSQTKHHWWW KLHFVWERVK ILRDYAGLIL FLEEDHYLAP DFYHVFKKMW KLKQQECPEC DVLSLGTYSA SRSFYGMADK VDVKTWKSTE HNMGLALTRN AYQKLIECTD TFCTYDDYNW DWTLQYLTVS CLPKFWKVLV PQIPRIFHAG DCGMHHKKTC
RPSTQSAQIE SLLNNNKQYM FPETLTISEK FTVVAISPPR KNGGWGDIRD HELCKSYRRL QHHHHHH.
Product Science Overview
MGAT2 has three characteristic domains typical of glycosyltransferases:
MGAT2 catalyzes the transfer of N-acetylglucosamine (GlcNAc) onto the free terminal mannose moiety in the core structure of the nascent N-linked glycan chain. This reaction is essential for the formation of the second branch in complex glycans . The enzyme’s activity is crucial for the proper functioning of glycoproteins, which are involved in various biological processes, including cell-cell communication, immune response, and protein stability .
The recombinant form of MGAT2 can be produced in different expression systems, including Escherichia coli and Sf9 Baculovirus cells. The recombinant MGAT2 produced in Sf9 cells is a single, glycosylated polypeptide chain containing 427 amino acids and has a molecular mass of approximately 49.3 kDa . This recombinant enzyme is often tagged with a His-tag to facilitate purification using chromatographic techniques .
Recombinant MGAT2 is widely used in laboratory research to study glycosylation processes and to understand the role of N-glycans in various biological functions. It is also used in the development of therapeutic glycoproteins and in the study of diseases related to glycosylation defects, such as carbohydrate-deficient glycoprotein syndrome, type II .
