MFGE8 Mouse

Milk Fat Globule-EGF Factor 8 Protein Mouse Recombinant
Cat. No.
BT2806
Source
Sf9, Baculovirus cells.
Synonyms

Milk fat globule-EGF factor 8 protein, isoform CRA_a, Putative uncharacterized protein, Mfge8, mCG_6301.

Appearance
Sterile filtered colorless solution.
Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MFGE8 Mouse Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 413 amino acids (23-426a.a.) and having a molecular mass of 46kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).
MFGE8 is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Milk fat globule-EGF factor 8 protein (Mfge8), also known as lactadherin, is a multifunctional secreted glycoprotein. It plays crucial roles in various biological processes, including: - Mammary gland development (morphogenesis) - Formation of new blood vessels (angiogenesis) - Progression of tumor growth - Maintaining tissue equilibrium (homeostasis) - Suppressing inflammation Mfge8 acts as a link between a molecule found on dying cells (phosphatidylserine) and a receptor on immune cells (Integrin alpha V beta 3). This interaction facilitates the removal of dead and dying cells, contributing to tissue maintenance.
Description
Recombinant MFGE8 Mouse protein, expressed in Sf9 insect cells using a baculovirus system, is a single-chain polypeptide. It undergoes glycosylation, a process of adding sugar molecules, and consists of 413 amino acids (specifically, amino acids 23 to 426). This protein has a molecular weight of 46 kDa, but on SDS-PAGE analysis, it appears as a band around 40-57 kDa. This discrepancy in size is due to glycosylation. For purification and later applications, a 6 amino acid His-tag is added at the C-terminus of the protein. The purification process utilizes proprietary chromatographic techniques to ensure high purity.
Physical Appearance
A clear and colorless solution that has been sterilized by filtration.
Formulation
The provided MFGE8 protein solution has a concentration of 0.25mg/ml. It is formulated in a Phosphate Buffered Saline (PBS) solution with a pH of 7.4 and supplemented with 10% glycerol.
Stability
For short-term storage (up to 4 weeks), keep the vial refrigerated at 4°C. For extended storage, freeze the solution at -20°C. To ensure optimal protein stability during long-term storage, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of this MFGE8 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms

Milk fat globule-EGF factor 8 protein, isoform CRA_a, Putative uncharacterized protein, Mfge8, mCG_6301.

Source
Sf9, Baculovirus cells.
Amino Acid Sequence

ADLASGDFCD SSLCLNGGTC LTGQDNDIYC LCPEGFTGLV CNETERGPCS PNPCYNDAKC LVTLDTQRGD IFTEYICQCP VGYSGIHCET GCSTQLGMEG GAIADSQISA SSVYMGFMGL QRWGPELARL YRTGIVNAWT ASNYDSKPWI QVNLLRKMRV SGVMTQGASR AGRAEYLKTF KVAYSLDGRK FEFIQDESGG DKEFLGNLDN NSLKVNMFNP TLEAQYIKLY PVSCHRGCTL RFELLGCELH GCSEPLGLKN NTIPDSQMSA SSSYKTWNLR AFGWYPHLGR LDNQGKINAW TAQSNSAKEW LQVDLGTQRQ VTGIITQGAR DFGHIQYVAS YKVAHSDDGV QWTVYEEQGS SKVFQGNLDN NSHKKNIFEK PFMARYVRVL PVSWHNRITL RLELLGCHHH HHH.

Product Science Overview

Introduction

Milk Fat Globule-EGF Factor 8 (MFG-E8), also known as lactadherin, is a glycoprotein that plays a crucial role in various cellular processes, including cell signaling, apoptosis, and phagocytosis . The recombinant form of MFG-E8 derived from mouse models is particularly valuable for researchers studying its biological functions and therapeutic potential.

Structure and Function

MFG-E8 is characterized by its unique structure, which includes two epidermal growth factor (EGF)-like domains and a discoidin-like domain. The EGF-like domains are responsible for binding to integrins, while the discoidin-like domain binds to phosphatidylserine on apoptotic cells . This dual binding capability allows MFG-E8 to mediate the clearance of apoptotic cells by phagocytes, thereby maintaining tissue homeostasis and preventing inflammation .

Biological Roles
  1. Phagocytosis and Apoptosis: MFG-E8 facilitates the recognition and engulfment of apoptotic cells by macrophages, promoting the clearance of dying cells and preventing the release of inflammatory substances .
  2. Angiogenesis: MFG-E8 promotes the formation of new blood vessels, which is essential for tissue repair and regeneration .
  3. Immune Regulation: MFG-E8 has been shown to modulate immune responses, reducing inflammation and improving outcomes in various disease models .
Therapeutic Potential

The recombinant form of MFG-E8 has shown promise in several therapeutic applications:

  • Inflammatory Diseases: By enhancing the clearance of apoptotic cells, MFG-E8 can reduce inflammation and improve prognosis in conditions such as sepsis and acute lung injury .
  • Cardiovascular Diseases: MFG-E8 has been identified as a potential biomarker and therapeutic target for heart failure and cardiac hypertrophy .
  • Cancer: MFG-E8’s role in angiogenesis makes it a potential target for cancer therapy, as inhibiting its function could reduce tumor growth and metastasis .

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