METRNL Human
Meteorin-like protein, Subfatin, METRNL.
Greater than 95.0% as determined by SDS-PAGE.
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Description
METRNL Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 46-311) containing 276 amino acids including a 10 a.a N-terminal His tag. The total molecular mass is 31.2kDa (calculated).
Product Specs
Known also as Meteorin-like protein, METRNL is a hormone that is secreted in response to physical activity (by skeletal muscle) or cold exposure (by adipose tissue). This hormone has been shown to increase energy expenditure, promote the browning of white fat, and improve glucose tolerance. METRNL achieves these effects by stimulating the migration of various immune cells into adipose tissue, where they initiate processes that enhance thermogenesis.
Recombinant Human METRNL, produced in E. coli, is a single, non-glycosylated polypeptide chain. This chain comprises 276 amino acids (a.a 46-311), including a 10 a.a N-terminal His tag, resulting in a calculated molecular mass of 31.2kDa.
The METRNL protein undergoes filtration (0.4µm) and lyophilization from a solution of 0.5mg/ml in 50mM acetate buffer (pH 4) containing 5% (w/v) trehalose and 1mM DTT.
To prepare a working stock solution of approximately 0.5mg/ml, it is recommended to reconstitute the lyophilized pellet in 0.1M acetate buffer (pH 4) and allow for complete dissolution.
Purity is determined to be greater than 95.0% by SDS-PAGE analysis.
Meteorin-like protein, Subfatin, METRNL.
MKHHHHHHAS QYSSDRCSWK GSGLTHEAHR KEVEQVYLRC AAGAVEWMYP TGALIVNLRP NTFSPARHLT VCIRSFTDSS GANIYLEKTG ELRLLVPDGD GRPGRVQCFG LEQGGLFVEA TPQQDIGRRT TGFQYELVRR HRASDLHELS APCRPCSDTE VLLAVCTSDF AVRGSIQQVT HEPERQDSAI HLRVSRLYRQ KSRVFEPVPE GDGHWQGRVR TLLECGVRPG HGDFLFTGHM HFGEARLGCA PRFKDFQRMY RDAQERGLNP CEVGTD.
Product Science Overview
Meteorin-like protein (METRNL), also known as Subfatin, Cometin, and Interleukin 39, is a secreted protein that has garnered significant interest due to its diverse roles in various physiological processes. This protein is encoded by the METRNL gene located on human chromosome 17 (17q25.3) and consists of 311 amino acids, including a 45 amino acid signal peptide .
Unlike Meteorin, which is predominantly expressed in the central nervous system, METRNL has a broader distribution. It is highly expressed in adipocytes, endothelial cells, activated monocytes, and myocytes . Additionally, METRNL is found in barrier tissues such as the skin and mucosal tissues of the digestive and respiratory tracts . The protein is secreted mainly by the endothelium, and its levels in circulation are significantly reduced when endothelial-specific knockout of METRNL is performed .
METRNL plays a crucial role in regulating glucose and lipid metabolism, maintaining cardiovascular system homeostasis, and promoting anti-inflammatory responses . It is involved in various physiological processes, including thermogenesis in brown/beige adipocytes and the production of anti-inflammatory molecules . METRNL also induces the phosphorylation of STAT3, a transcription factor involved in various cellular processes .
METRNL has emerged as a promising biomarker and therapeutic target for cardiovascular and metabolic diseases. Its predictive and therapeutic roles have been studied in conditions such as atherosclerosis, ischemic heart disease, cardiac remodeling, heart failure, hypertension, chemotherapy-induced myocardial injury, diabetes mellitus, and obesity . The decline in circulating METRNL levels is closely associated with the occurrence of these diseases .
Recombinant human METRNL is produced using Chinese Hamster Ovary (CHO) cell lines and is available in carrier-free formulations . The recombinant protein is used in various research applications, including studies on its bioactivity, such as inducing STAT3 phosphorylation in embryonic rat cortical neurons . The protein is typically lyophilized and reconstituted in PBS for experimental use .
