METRN Mouse
Meteorin Mouse Recombinant
Cat. No.
BT24116
Source
Sf9,
Baculovirus cells.
Synonyms
Meteorin, Hypoxia/reoxygenation regulatory factor, Metrn,
1810034B16Rik, Hyrac.
Appearance
Sterile Filtered colorless solution.
Purity
Greater
than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
METRN produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 276 amino acids (22-291 a.a.) and
having a molecular mass of 30.2kDa (Migrates at 28-40kDa on SDS-PAGE under
reducing conditions).
METRN is expressed with a 6 amino acid His tag at
C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Meteorin (METRN) plays a crucial role in neurogenesis, contributing to both glial cell differentiation and the formation of axonal networks. It promotes the differentiation of astrocytes and can induce the transformation of cerebellar astrocytes into radial glia. Additionally, METRN stimulates axonal extension in specific neurons within sensory ganglia by activating neighboring satellite glia.
Description
METRN, produced using Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain comprising 276 amino acids (specifically, amino acids 22-291). It possesses a molecular mass of 30.2 kDa. Under reducing conditions on SDS-PAGE, it migrates at a range of 28-40 kDa. The protein is expressed with a 6-amino acid His tag located at the C-terminus. Purification is achieved through proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, filtered solution that is colorless.
Formulation
The METRN protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product should be stored at 4°C. For longer-term storage, it is recommended to freeze the product at -20°C. To further ensure stability during long-term storage, consider adding a carrier protein such as 0.1% HSA or BSA. It is important to avoid repeated cycles of freezing and thawing.
Purity
The purity of the METRN protein is greater than 90.0%, as determined by SDS-PAGE analysis.
Synonyms
Meteorin, Hypoxia/reoxygenation regulatory factor, Metrn,
1810034B16Rik, Hyrac.
Source
Sf9,
Baculovirus cells.
Amino Acid Sequence
GYSEDRCSWR
GSGLTQEPGS VGQLTLDCTE GAIEWLYPAG ALRLTLGGPD PGTRPSIVCL RPERPFAGAQ VFAERMTGNL
ELLLAEGPDL AGGRCMRWGP RERRALFLQA TPHRDISRRV AAFRFELHED QRAEMSPQAQ GLGVDGACRP
CSDAELLLAA CTSDFVIHGT IHGVAHDTEL QESVITVVVA RVIRQTLPLF KEGSSEGQGR ASIRTLLRCG
VRPGPGSFLF MGWSRFGEAW LGCAPRFQEF SRVYSAALTT HLNPCEMALD HHHHHH.
Product Science Overview
Structure and Characteristics
- Molecular Weight: Meteorin has a predicted molecular mass of approximately 30 kDa .
- Amino Acid Sequence: The recombinant mouse Meteorin protein is derived from the mouse myeloma cell line, NS0, and includes the amino acids Gly22-Asp291 with a C-terminal 6-His tag .
- Purity: The protein is highly purified, with a purity level greater than 90%, as determined by SDS-PAGE visualized with silver staining and quantitative densitometry by Coomassie® Blue staining .
- Endotoxin Level: The endotoxin level is less than 0.10 EU per 1 μg of the protein, as measured by the LAL method .
Biological Activity
Applications
Recombinant mouse Meteorin protein is used in various research applications, including:
- Neuroscience Research: Due to its role in glial cell differentiation and neurite outgrowth, Meteorin is valuable in studies related to neural development and regeneration.
- Cell Culture: The protein is often used in cell culture to study its effects on glial and neuronal cells.
- Hypoxia/Reoxygenation Studies: Meteorin’s role as a hypoxia/reoxygenation regulatory factor makes it relevant in research focused on cellular responses to oxygen deprivation and subsequent reoxygenation.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
