MEP1B Mouse
Description
|
MEP1B produced in Sf9
Insect cells is a single, glycosylated polypeptide chain containing 642 amino
acids (21-654aa) and having a molecular mass of 72.6kDa. (Molecular size on
SDS-PAGE will appear at approximately 70-100kDa). MEP1Bis expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques. |
Product Specs
| Produced in Sf9 Insect cells, MEP1B is a single, glycosylated polypeptide chain comprising 642 amino acids (21-654aa) with a molecular weight of 72.6kDa. It's important to note that the molecular size on SDS-PAGE will appear approximately between 70-100kDa. The protein is expressed with an 8 amino acid His tag at the C-Terminus and purified using proprietary chromatographic techniques. |
| The MEP1B protein solution is provided at a concentration of 0.25mg/ml. It is formulated in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
Product Science Overview
Meprin β is a membrane-bound metalloprotease that forms disulfide-linked homo- or heterooligomers with meprin α subunits . The recombinant mouse meprin β protein is typically expressed in baculovirus systems and purified to high levels of purity (>90%) for research purposes . The protein is often tagged with a His tag at the C-terminus to facilitate purification and detection .
Meprin β exhibits a strong preference for acidic amino acids at the P1’ position and is known to cleave a variety of substrates, including:
- Fibroblast Growth Factor 19 (FGF19)
- Vascular Endothelial Growth Factor A (VEGFA)
- Interleukin-1 Beta (IL1B)
- Interleukin-18 (IL18)
- Procollagen I and III
- E-cadherin
- Kallikrein-related peptidases (KLK7)
- Gastrin
- ADAM10
- Tenascin-C
The presence of several pro-inflammatory cytokines among its substrates implicates meprin β in inflammation. Additionally, its ability to degrade extracellular matrix components suggests a role in tissue remodeling .
Recombinant mouse meprin β protein is widely used in research to study its role in various biological processes. It is particularly useful in investigating its involvement in inflammation, tissue remodeling, and protein shedding. The recombinant protein is also employed in assays to identify potential inhibitors and to understand its substrate specificity .
