MEP1A Human

Meprin A Subunit Alpha Human Recombinant

Cat. No.

BT23957

Source

Sf9, Baculovirus cells.

Synonyms

Meprin A Subunit Alpha, PABA Peptide Hydrolase, N-Benzoyl-L-Tyrosyl-P-Amino-Benzoic Acid Hydrolase Subunit Alpha, Meprin A, Alpha (PABA Peptide Hydrolase), Endopeptidase-2, EC 3.4.24.18, PPH Alpha, BA268F1.1 (Meprin A Alpha (PABA Peptide Hydrolase)), EC 3.4.24, PPHA, MEP1A.

Appearance

Sterile filtered colorless solution.

Purity

Greater than 85.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MEP1A produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 589 amino acids (22-601 a.a.) and having a molecular mass of 67.4kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa). MEP1A is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction

Meprin A subunit alpha (MEP1A), a member of the peptidase M12A family, is a single-pass type I membrane protein. Its expression is abundant in kidney and intestinal epithelial cells, and it is secreted into the urinary tract and intestinal lumen. Additionally, MEP1A is present in leukocytes and, under specific conditions, in cancer cells. This protein plays a role in inflammatory processes and possesses the ability to proteolytically degrade extracellular matrix proteins and process bioactive proteins.

Description

Produced in Sf9 Baculovirus cells, MEP1A is a single, glycosylated polypeptide chain with a molecular weight of 67.4 kDa. It comprises 589 amino acids (22-601 a.a.). On SDS-PAGE, its molecular size appears between 70-100 kDa. The protein is expressed with a 6 amino acid His tag at the C-terminus and undergoes purification using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution, sterile-filtered.

Formulation

The MEP1A protein solution (0.25 mg/ml) is prepared in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.

Stability

For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freezing at -20°C is recommended. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freezing and thawing cycles.

Purity

Purity exceeds 85.0% as assessed by SDS-PAGE.

Synonyms

Source

Sf9, Baculovirus cells.

Amino Acid Sequence

ADPVPIKYLP EENVHDADFG EQKDISEINL AAGLDLFQGD ILLQKSRNGL RDPNTRWTFP IPYILADNLG LNAKGAILYA FEMFRLKSCV DFKPYEGESS YIIFQQFDGC WSEVGDQHVG QNISIGQGCA YKAIIEHEIL HALGFYHEQS RTDRDDYVNI WWDQILSGYQ HNFDTYDDSL ITDLNTPYDY ESLMHYQPFS FNKNASVPTI TAKIPEFNSI IGQRLDFSAI DLERLNRMYN CTTTHTLLDH CTFEKANICG MIQGTRDDTD WAHQDSAQAG EVDHTLLGQC TGAGYFMQFS TSSGSAEEAA LLESRILYPK RKQQCLQFFY KMTGSPSDRL VVWVRRDDST GNVRKLVKVQ TFQGDDDHNW KIAHVVLKEE QKFRYLFQGT KGDPQNSTGG IYLDDITLTE TPCPTGVWTV RNFSQVLENT SKGDKLQSPR FYNSEGYGFG VTLYPNSRES SGYLRLAFHV CSGENDAILE WPVENRQVII TILDQEPDVR NRMSSSMVFT TSKSHTSPAI NDTVIWDRPS RVGTYHTDCN CFRSIDLGWS GFISHQMLKR RSFLKNDDLI IFVDFEDITH LSQHHHHHH.

Product Science Overview

Introduction

Meprin A subunit alpha, also known as MEP1A, is a protein-coding gene that plays a crucial role in various biological processes. It is part of the meprin A complex, which is involved in proteolysis, the breakdown of proteins into smaller polypeptides or amino acids. This article provides a detailed overview of the background, structure, function, and significance of Meprin A subunit alpha, particularly focusing on its human recombinant form.

Gene and Protein Structure

The MEP1A gene encodes the Meprin A subunit alpha protein. This protein is a single-pass type I membrane protein that belongs to the peptidase M12A family. The gene is located on chromosome 6 in humans and is known for its metalloendopeptidase activity, which is essential for the degradation of extracellular matrix proteins.

The Meprin A subunit alpha protein contains several important domains:

EGF-like domain: Involved in protein-protein interactions.
MAM domain: Plays a role in cell adhesion and signaling.
MATH domain: Involved in protein-protein interactions and ubiquitination processes.

Biological Functions

Meprin A subunit alpha is primarily expressed in kidney and intestinal epithelial cells. It is secreted into the urinary tract and intestinal lumen and can also be found in leukocytes and cancer cells under certain conditions. The protein is capable of proteolytically degrading extracellular matrix proteins and processing bioactive proteins, which makes it significant in various physiological and pathological processes.

Recombinant Form

The recombinant form of Meprin A subunit alpha is produced using DNA sequences encoding the human MEP1A extracellular domain. This recombinant protein is often fused with a polyhistidine tag at the C-terminus to facilitate purification. The recombinant human Meprin A subunit alpha is expressed in HEK293 cells and is typically purified to a high degree of purity (>90%) as determined by SDS-PAGE.

Applications and Significance

Recombinant Meprin A subunit alpha has several applications in research and medicine:

Proteolysis Studies: Used to study the mechanisms of protein degradation and processing.
Disease Research: Investigated for its role in diseases such as rectosigmoid junction neoplasm and sigmoid neoplasm.
Drug Development: Potential target for developing therapeutic agents aimed at modulating its activity in various diseases.

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MEP1A Human

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