MEK1 Human

Mitogen Activated Kinase Kinase 1 Human Recombinant
Cat. No.
BT11318
Source
Sf9 Insect Cells.
Synonyms
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAP2K1 active Human Recombinant produced in Sf9 cells is a glycosylated, polypeptide chain containing amino acids 2-393 having a molecular mass of 47 kDa. MAP2K1 is fused to a polyhistidine tag and is purified by proprietary chromatographic techniques.

Product Specs

Introduction
MAP2K1, a member of the dual specificity protein kinase family, functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), integrate multiple biochemical signals. MEK1, positioned upstream of MAP kinases, activates their enzymatic activity in response to a wide range of extra- and intracellular signals. As a crucial component of the MAP kinase signal transduction pathway, MEK1 participates in various cellular processes, including proliferation, differentiation, transcription regulation, and development. MAP2K1 catalyzes the simultaneous phosphorylation of threonine and tyrosine residues within a thr-glu-tyr sequence found in map kinases. MEK1 activates erk1 and erk2 map kinases.
Description
Active human recombinant MAP2K1, produced in Sf9 cells, is a glycosylated polypeptide chain comprising amino acids 2-393. With a molecular mass of 47 kDa, it is fused to a polyhistidine tag and purified using proprietary chromatographic techniques.
Physical Appearance
Clear, sterile-filtered solution.
Formulation
MEK1 is supplied in a solution containing 40mM Tris (pH 8), 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate, and 0.03% Brij-35.
Biological Activity
The activity of MEK1 ranges from approximately 125 to 175 units per mg. One unit of MEK1 activity is defined as the amount required to transfer 1 nanomole of phosphate to ERK1/2 peptide per minute at 30 degrees Celsius in a reaction containing 100 micromolar ATP. Recombinant active MEK1 can also phosphorylate ERK1, ERK2, and GSK-3β. It's important to note that kinase activity might vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.
Stability
For short-term storage (1-2 weeks), keep at 4 degrees Celsius. For long-term storage, freeze at -20 degrees Celsius. Minimize freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Source
Sf9 Insect Cells.

Product Science Overview

Introduction

Mitogen-Activated Kinase Kinase 1 (MAP2K1), also known as MEK1, is a crucial component of the MAPK/ERK signaling pathway. This pathway is essential for regulating various cellular processes, including proliferation, differentiation, transcription regulation, and development . The human recombinant form of MAP2K1 is widely used in research to study these processes and to develop potential therapeutic interventions.

Structure and Function

MAP2K1 is a serine/threonine-specific protein kinase. It is activated by phosphorylation at specific serine residues (Ser218 and Ser222) by upstream kinases such as Raf1 . Upon activation, MAP2K1 phosphorylates and activates downstream kinases, primarily ERK1 and ERK2, which then translocate to the nucleus to regulate gene expression .

Role in Cellular Processes

The MAPK/ERK pathway, in which MAP2K1 plays a pivotal role, is involved in various cellular responses to external stimuli. These include:

  • Proliferation: MAP2K1 activation leads to cell division and growth.
  • Differentiation: It helps cells develop into their specialized forms.
  • Transcription Regulation: MAP2K1 influences the transcription of specific genes.
  • Development: It is crucial for the proper development of organisms .
Clinical Significance

Mutations in the MAP2K1 gene can lead to several disorders, including Noonan Syndrome and cardiofaciocutaneous syndrome . These conditions are characterized by developmental abnormalities and various other symptoms. Understanding the function and regulation of MAP2K1 is therefore critical for developing targeted therapies for these conditions.

Research Applications

Human recombinant MAP2K1 is extensively used in research to:

  • Study the MAPK/ERK signaling pathway.
  • Investigate the molecular mechanisms underlying various diseases.
  • Develop and test potential therapeutic agents targeting this pathway .

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THE BIOTEK
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