MEIS3 Human

The MEIS3 protein has several isoforms, with the most common being a 375 amino acid isoform that weighs approximately 41 kDa . The recombinant version of this protein is often produced in Escherichia coli (E. coli) and includes an N-terminal His-tag for purification purposes . The amino acid sequence of the recombinant MEIS3 protein typically corresponds to the first 358 amino acids of the human MEIS3 protein .

While the exact functions of MEIS3 are still being elucidated, it is known to play a role in the regulation of transcription, DNA-dependent processes, and various signaling pathways. MEIS3 has been implicated in the negative regulation of apoptotic processes and the positive regulation of the protein kinase B signaling cascade . Additionally, MEIS3 is involved in the development of the nervous system and has been studied in relation to neuronitis .

Recombinant MEIS3 protein is primarily used in research settings to study its role in gene regulation and developmental processes. It is often utilized in Western Blot (WB) or imaging assays due to its denatured state . The protein’s involvement in various signaling pathways makes it a valuable tool for understanding the molecular mechanisms underlying cell differentiation and development.

For optimal stability, the recombinant MEIS3 protein should be stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s integrity .