MECOM Human

MDS1 And EVI1 Complex Locus Human Recombinant
Cat. No.
BT23602
Source
Escherichia Coli.
Synonyms
MDS1 And EVI1 Complex Locus, Ecotropic Virus Integration Site 1 Protein Homolog, Myelodysplasia Syndrome-Associated Protein 1, AML1-EVI-1, MDS1-EVI1, EVI1, MDS1, PRDM3, AML1-EVI-1 Fusion Protein, MDS1 And EVI1 Complex Locus Protein EVI1, MDS1 And EVI1 Complex Locus Protein MDS1, Oncogene EVI1, Zinc Finger Protein Evi1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MECOM Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids (1-169 a.a) and having a molecular mass of 21.1kDa.
MECOM is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
MDS1, a transcriptional regulator and oncoprotein, plays a crucial role in various cellular processes such as hematopoiesis, development, cell differentiation, apoptosis, and proliferation. It interacts with several proteins, including CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. Notably, MDS1 overexpression due to translocation with the AML1 gene has been linked to leukemia development. Multiple transcript variants encoding different isoforms of MDS1 have been identified.
Description
Recombinant MECOM protein, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 192 amino acids (specifically, amino acids 1-169) and exhibits a molecular weight of 21.1 kDa. The MECOM protein is fused to a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear solution that has undergone sterile filtration.
Formulation
The MECOM protein solution has a concentration of 0.25 mg/ml and is prepared in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), the MECOM protein should be kept at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.
Purity
The purity of the MECOM protein is greater than 85%, as assessed by SDS-PAGE analysis.
Synonyms
MDS1 And EVI1 Complex Locus, Ecotropic Virus Integration Site 1 Protein Homolog, Myelodysplasia Syndrome-Associated Protein 1, AML1-EVI-1, MDS1-EVI1, EVI1, MDS1, PRDM3, AML1-EVI-1 Fusion Protein, MDS1 And EVI1 Complex Locus Protein EVI1, MDS1 And EVI1 Complex Locus Protein MDS1, Oncogene EVI1, Zinc Finger Protein Evi1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMRSKGRA RKLATNNECV YGNYPEIPLE EMPDADGVAS TPSLNIQEPC SPATSSEAFT PKEGSPYKAP IYIPDDIPIP AEFELRESNM PGAGLGIWTK RKIEVGEKFG PYVGEQRSNL KDPSYGWEVH LPRSRRVSVH SWLYLGKRSS DVGIAFSQAD VYMPGLQCAF LS

Product Science Overview

Structure and Function

The MECOM locus is located on the long arm of chromosome 3 at position 3q26.2 . It includes several alternative transcripts, with EVI1 being a prominent oncogenic zinc-finger transcription factor. EVI1 is known for its role in myeloid malignancies, where its overexpression contributes to disease progression and poor clinical outcomes .

EVI1 exists in two main forms:

  1. EVI1: The shorter isoform, which is abundant and oncogenic.
  2. MDS1-EVI1: The longer isoform, created by splicing the MDS1 gene upstream to EVI1 .

EVI1 contains ten zinc fingers arranged in two sets: seven in the N-terminal and three in the C-terminal. These zinc fingers allow EVI1 to bind DNA and regulate gene expression .

Role in Hematopoiesis

The MDS1-EVI1 (ME) isoform is critical for the long-term function of hematopoietic stem cells (HSCs). Studies have shown that ME is exclusively expressed in the stem cell compartment and is essential for maintaining the quiescence and long-term repopulation capacity of HSCs . ME deficiency leads to a reduction in HSC numbers and a shift from quiescence to active cycling, which can result in hematopoietic defects .

Implications in Leukemogenesis

The dysregulation of EVI1 is strongly associated with leukemogenesis. Overexpression of EVI1 is linked to poor outcomes in myeloid malignancies, including acute myeloid leukemia (AML). The oncogenic properties of EVI1 are attributed to its ability to disrupt normal gene expression and promote uncontrolled cell proliferation .

Therapeutic Potential

Understanding the biology of MECOM and its associated transcripts has significant implications for developing targeted therapies. Current research is focused on identifying novel therapeutic interventions that can modulate EVI1 activity and improve clinical outcomes for patients with myeloid malignancies .

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