Introduction
Malate dehydrogenase (MDH, EC 1.1.1.37) is an enzyme that plays a crucial role in the citric acid cycle and gluconeogenesis. It catalyzes the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. MDH is distinct from malic enzyme, which catalyzes a different reaction involving pyruvate and NADPH. In the citric acid cycle, MDH facilitates the conversion of malate to oxaloacetate, a key step in energy production. In gluconeogenesis, MDH helps shuttle oxaloacetate from the mitochondria to the cytoplasm, where it is converted to glucose.
Description
This product consists of Malate Dehydrogenase (MDH1) derived from chicken heart. The encoding gene for MDH1 was cloned from a chicken heart cDNA library. After expression, the enzyme is purified using advanced chromatographic methods to ensure high purity.
Physical Appearance
Provided as a sterile powder that has been freeze-dried.
Formulation
The composition of the lyophilized powder includes 0.59mg of NaPO4 per milligram of protein.
Solubility
To reconstitute the lyophilized MDH, it is recommended to dissolve it in sterile water with a resistance of 18 megaohms-centimeter (18MΩ-cm).
Stability
The lyophilized MDH remains stable for up to 3 weeks at room temperature. However, for long-term storage, it is recommended to store the lyophilized enzyme below -18°C in a dry environment. After reconstitution, the enzyme can be stored at 4°C for 2 to 7 days. For extended storage, it is advisable to keep the reconstituted MDH below -18°C. Repeated freezing and thawing of the enzyme should be avoided.
Purity
The purity of this product is greater than 95%, as determined by two methods: (a) Reverse-phase high-performance liquid chromatography (RP-HPLC) and (b) Sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Unit Definition
One unit of enzyme activity is defined as the amount of enzyme that catalyzes the formation of 1 micromole of NAD+ per minute under standard assay conditions (25°C, pH 7.5).
Specific Activity
This MDH preparation exhibits a specific activity exceeding 710 units per milligram of protein.
Synonyms
Malate dehydrogenase cytoplasmic, EC 1.1.1.37, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375, MDH1.
