MCTS1 Human

Malignant T-Cell-Amplified Sequence 1 Human Recombinant

Cat. No.

BT23517

Source

E.coli.

Synonyms

MCT-1, MCT1, Malignant T-cell-amplified sequence 1, Multiple copies T-cell malignancies.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Shipped with Ice Packs

In Stock

Quick Inquiry

Description

MCTF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 204 amino acids (1-181 a.a.) and having a molecular mass of 22.9kDa. MCTF1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

MCTS1, also known as Malignant T cell amplified sequence 1, acts as an anti-oncogene involved in cell cycle regulation. It achieves this by reducing cell doubling time, promoting anchorage-dependent growth, and accelerating both G1 transit time and the G1/S transition. Additionally, MCTS1 functions as a translation enhancer by recruiting the density-regulated protein DENR and binding to the 5'-terminus cap complex of mRNAs. This interaction modifies the mRNA translation profile, leading to increased protein levels of BCL2L2, TFDP1, MRE11A, CCND1, and E2F1 without affecting their mRNA levels.

Description

Recombinant human MCTF1, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 204 amino acids, including amino acids 1-181 of the MCTF1 sequence, and has a molecular weight of 22.9 kDa. The protein is fused to a 23 amino acid His-tag at its N-terminus and purified using proprietary chromatographic techniques.

Physical Appearance

Clear, colorless solution that has been sterilized by filtration.

Formulation

The MCTF1 protein solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.15 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1% concentration) is advised for long-term storage. Avoid repeated freeze-thaw cycles to maintain protein integrity.

Purity

The purity of the protein is greater than 95% as determined by SDS-PAGE analysis.

Synonyms

MCT-1, MCT1, Malignant T-cell-amplified sequence 1, Multiple copies T-cell malignancies.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMFKKFDE KENVSNCIQL KTSVIKGIKN QLIEQFPGIE PWLNQIMPKK DPVKIVRCHE HIEILTVNGE LLFFRQREGP FYPTLRLLHK YPFILPHQQV DKGAIKFVLS GANIMCPGLT SPGAKLYPAA VDTIVAIMAE GKQHALCVGV MKMSAEDIEK VNKGIGIENI HYLNDGLWHM KTYK.

Product Science Overview

Gene and Protein Function

MCTS1 is a protein-coding gene that plays a crucial role in the regulation of the cell cycle. It decreases cell doubling time and promotes anchorage-dependent growth. The protein encoded by MCTS1 is involved in the initiation of translation, a process essential for protein synthesis. It promotes the recruitment of aminoacylated initiator tRNA to the P site of 40S ribosomes, facilitating the translation process.

Additionally, MCTS1 has been shown to increase the activity of CDK4 and CDK6 kinases and elevate the levels of CCND1/cyclin D1 protein, which are critical for the G1/S transition in the cell cycle. This makes MCTS1 a significant player in cell proliferation and growth.

Oncogenic Potential

The oncogenic potential of MCTS1 is highlighted by its amplification in malignant T-cell lymphomas. When constitutively expressed, MCTS1 can hyperactivate DNA damage signaling pathways, leading to increased phosphorylation of histone H2AX and the formation of damage foci. This suggests that MCTS1 may contribute to the genomic instability observed in cancer cells.

Recombinant MCTS1

Recombinant MCTS1 proteins are produced using various expression systems, including Escherichia coli (E. coli), yeast, and mammalian cells. These recombinant proteins are used in research to study the function of MCTS1 and its role in cancer. They are also utilized in various assays, including ELISA and Western blotting, to detect and quantify MCTS1 protein levels.

Recombinant MCTS1 proteins are typically tagged with purification tags such as His or GST to facilitate their purification and detection. These proteins are available in different forms, including full-length proteins and specific fragments, to suit various research needs.

Clinical Implications

Given its role in cell cycle regulation and oncogenesis, MCTS1 is a potential target for cancer therapy. Understanding the function and regulation of MCTS1 could lead to the development of novel therapeutic strategies aimed at targeting this protein in T-cell malignancies and other cancers.

Quick Inquiry

Personal Email Detected

Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Name*

Email*

Phone

Country

Product Name

Quantity&Size*

Message

MCTS1 Human

Description

Product Specs

Product Science Overview

Quick Inquiry

Category

Service