MCP 5 Mouse

Monocyte Chemotactic Protein-5 Mouse Recombinant (CCL12)
Cat. No.
BT18473
Source
Escherichia Coli.
Synonyms
C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible cytokine A12, Ccl12, Mcp5, Scya12.
Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

MCP-5 Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 82 amino acids and having a molecular mass of 9.3kDa.
The MCP5 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
MCP-5 (CCL12), a CC chemokine found in mice, shares significant similarity with human MCP1, exhibiting a 66% amino acid sequence identity in the mature protein. Constitutive expression of MCP5 is observed in the thymus and lymph nodes. Notably, CCL12 expression is upregulated in activated macrophages and mast cells under inflammatory conditions. The murine MCP1 gene is located on chromosome 11 within the CC chemokine cluster. Recombinant CCL12 exhibits potent chemoattractant properties for monocytes and lymphocytes, while neutrophils remain unresponsive. At elevated concentrations, MCP-5 can also induce chemotaxis in eosinophils. CCR2 serves as a functional receptor for CCL12.
Description
Recombinant MCP-5 Mouse, produced in E. coli, is a single, non-glycosylated polypeptide chain composed of 82 amino acids, resulting in a molecular weight of 9.3 kDa. The purification of MCP5 is achieved through proprietary chromatographic methods.
Physical Appearance
White lyophilized (freeze-dried) powder, sterile filtered.
Formulation
Lyophilized from a 0.2 µm filtered solution concentrated in 1xPBS, pH 7.4.
Solubility
To reconstitute the lyophilized MCP5, it is recommended to dissolve it in sterile 18 M-cm H₂O at a concentration not less than 100 µg/ml. This solution can be further diluted in other aqueous solutions as needed.
Stability
Lyophilized CCL12 demonstrates stability at room temperature for up to 3 weeks; however, it is recommended to store it desiccated below -18°C. Once reconstituted, MCP5 should be stored at 4°C for 2-7 days. For long-term storage, it is advisable to store it below -18°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 97.0%, as determined by: (a) RP-HPLC analysis. (b) SDS-PAGE analysis.
Biological Activity
The biological activity is assessed by the ability to induce chemotaxis in human peripheral blood monocytes within a concentration range of 10.0-50.0 ng/ml.
Synonyms
C-C motif chemokine 12, MCP-1-related chemokine, Monocyte chemoattractant protein 5, Monocyte chemotactic protein 5, MCP-5, Small-inducible cytokine A12, Ccl12, Mcp5, Scya12.
Source
Escherichia Coli.
Amino Acid Sequence
GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG.

Product Science Overview

Introduction

Monocyte Chemotactic Protein-5 (MCP-5), also known as Chemokine (C-C motif) ligand 12 (CCL12), is a small cytokine belonging to the CC chemokine family. It has been characterized in mice and is closely related to human MCP-1, sometimes referred to as MCP-1-related chemokine . MCP-5 plays a crucial role in the immune system by attracting monocytes, eosinophils, and lymphocytes to sites of inflammation, but not neutrophils .

Expression and Function

MCP-5 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, its expression is induced in activated macrophages and mast cells . This chemokine is involved in allergic inflammation and the host response to pathogens, playing a pivotal role during the early stages of allergic lung inflammation . MCP-5 signals through the CCR2 receptor, which is also used by other chemokines like MCP-1 .

Recombinant Production

Recombinant MCP-5 is typically produced in Escherichia coli (E. coli) expression systems. The recombinant protein is purified and lyophilized for use in various research applications. For instance, the recombinant murine MCP-5 is a 9.3 kDa protein containing 82 amino acid residues, including the four highly conserved cysteine residues present in the CC chemokines . The protein is purified to a high degree, with a purity of ≥ 98% as determined by SDS-PAGE gel and HPLC analyses .

Applications in Research

Recombinant MCP-5 is used in various research applications, including studies on chemotaxis, inflammation, and immune response. It is particularly useful in in vitro assays to study the chemotactic activity of monocytes and other immune cells . The ability of MCP-5 to attract specific cell types makes it a valuable tool for understanding the mechanisms of immune cell migration and the role of chemokines in disease processes.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.