MCP 4 Human, His

Monocyte Chemotactic Protein-4 (MCP-4), also known as CCL13, is a chemokine that plays a crucial role in the immune system by attracting monocytes, T lymphocytes, eosinophils, and basophils to sites of inflammation. This protein is part of the C-C motif chemokine family and is induced by inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α) .

Human recombinant MCP-4 is a non-glycosylated protein produced in Escherichia coli (E. coli). It consists of 96 amino acids, including a 21-amino acid His-Tag at the N-terminus, resulting in a molecular mass of approximately 10.8 kDa . The His-Tag facilitates purification and detection of the protein.

MCP-4 is a ligand for three G protein-coupled receptors: CCR2, CCR3, and CCR5 . Upon binding to these receptors, MCP-4 activates signaling pathways that lead to the chemotaxis of immune cells. This signaling is particularly associated with allergic responses and other inflammatory conditions .

The biological activity of MCP-4 is determined by its ability to chemoattract human monocytes. Typically, the effective dose (ED50) for this activity ranges from 7 to 75 ng/mL . This chemotactic property makes MCP-4 a valuable tool for studying immune responses and inflammation.

Recombinant MCP-4 is widely used in research to investigate the mechanisms of immune cell migration and the role of chemokines in inflammatory diseases. It is also utilized in various assays, including Western Blot, Immunohistochemistry, and ELISA .

Lyophilized MCP-4 should be stored at -20°C for long-term stability. Upon reconstitution with sterile water, it is recommended to aliquot and store the protein at -20°C to avoid repeated freeze-thaw cycles . Adding a carrier protein, such as bovine serum albumin (BSA), can enhance stability during storage.