MCAM Human
Sf9, Insect cells.
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18, Cell Surface Glycoprotein MUC18, Melanoma Adhesion Molecule, CD146 Antigen, CD146, Melanoma Cell Adhesion Molecule, S-Endo 1 Endothelial-Associated Antigen, Melanoma-Associated Antigen MUC18, Cell Surface Glycoprotein P1H12, Melanoma-Associated Antigen A32, Gicerin.
Sterile filtered colorless solution.
Greater than 95.0% as determined by SDS-PAGE.
Description
MCAM produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 547 amino acids (24-559 a.a.) and having a molecular mass of 61.0kDa (Molecular size on SDS-PAGE will appear at approximately 70-100kDa).
MCAM is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The cell surface glycoprotein MUC18, also known as MCAM, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. MCAM is linked to several types of carcinomas, playing a role in tumor progression and metastasis. Moreover, it is involved in embryonic neural development. Additionally, MCAM participates in cell adhesion and helps maintain the cohesion of the endothelial monolayer at intercellular junctions within vascular tissue.
Produced in Sf9 insect cells, MCAM is a single, glycosylated polypeptide chain comprising 547 amino acids (specifically, amino acids 24 to 559). It possesses a molecular mass of 61.0 kDa. However, on SDS-PAGE, its apparent molecular size will range from approximately 70 kDa to 100 kDa. This MCAM variant is expressed with a 6-amino acid His tag located at the C-terminus. Purification is achieved using proprietary chromatographic techniques.
The product is a sterile-filtered solution that is colorless.
The MCAM protein solution has a concentration of 0.5 mg/ml. It is prepared in Phosphate Buffered Saline (PBS) with a pH of 7.4 and contains 10% glycerol.
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it should be frozen at -20°C. To ensure optimal stability during long-term storage, the addition of a carrier protein (either 0.1% HSA or BSA) is recommended. It is important to avoid repeated cycles of freezing and thawing.
Analysis by SDS-PAGE indicates a purity greater than 95.0%.
Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18, Cell Surface Glycoprotein MUC18, Melanoma Adhesion Molecule, CD146 Antigen, CD146, Melanoma Cell Adhesion Molecule, S-Endo 1 Endothelial-Associated Antigen, Melanoma-Associated Antigen MUC18, Cell Surface Glycoprotein P1H12, Melanoma-Associated Antigen A32, Gicerin.
Sf9, Insect cells.
VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGAAAL EHHHHHH.
Product Science Overview
Melanoma Cell Adhesion Molecule (MCAM), also known as CD146 or MUC18, is a heavily glycosylated transmembrane protein. It was initially identified as a marker of melanoma metastasis and has since been implicated in various physiological and pathological processes, including cell adhesion, migration, and signaling .
MCAM is a member of the immunoglobulin superfamily and consists of five immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail. The protein is expressed in a variety of cell types, including endothelial cells, smooth muscle cells, and certain subsets of T lymphocytes . In the context of melanoma, MCAM expression is significantly upregulated in advanced primary melanoma and metastatic lesions, but it is rarely found in benign naevi or normal melanocytes .
MCAM plays a crucial role in cell-cell and cell-matrix interactions. It mediates the adhesion of melanoma cells to endothelial cells, facilitating their extravasation and subsequent metastasis . Additionally, MCAM is involved in the recruitment of encephalitogenic T lymphocytes to the central nervous system, highlighting its role in neuroinflammatory conditions such as multiple sclerosis .
The expression of MCAM in melanoma is associated with poor prognosis and increased metastatic potential. As a result, MCAM has been explored as a potential biomarker for melanoma progression and a target for therapeutic intervention . In multiple sclerosis, the blockade of MCAM or depletion of MCAM-positive T lymphocytes has been shown to reduce the severity of experimental autoimmune encephalomyelitis, suggesting its potential as a therapeutic target in neuroinflammatory diseases .
Human recombinant MCAM is produced using recombinant DNA technology, which involves the insertion of the MCAM gene into a suitable expression system, such as bacteria, yeast, or mammalian cells. This allows for the large-scale production of MCAM for research and therapeutic purposes. Recombinant MCAM can be used in various applications, including the study of cell adhesion mechanisms, the development of MCAM-targeted therapies, and the investigation of its role in disease processes.
