MBIP Human
MAP3K12 Binding Inhibitory Protein 1 Human Recombinant
Cat. No.
BT23034
Source
Escherichia Coli.
Synonyms
MAP3K12 binding inhibitory protein 1, MAPK upstream kinase-binding inhibitory protein, MUK-binding inhibitory protein, MBIP.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
MBIP Human Recombinant produced in E. coli is a single polypeptide chain containing 367 amino acids (1-344) and having a molecular mass of 41.7kDa. MBIP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
MBIP (MAP3K12 Binding Inhibitory Protein 1) is a component of the ATAC complex, which comprises histones H3 and H4 and exhibits histone acetyltransferase activity. By inhibiting MAP3K12 activity, MBIP triggers the activation of the JNK/SAPK signaling pathway.
Description
Recombinant human MBIP, expressed in E. coli, is a single polypeptide chain consisting of 367 amino acids (residues 1-344) with a molecular weight of 41.7 kDa. The protein includes a 23-amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The MBIP solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4 M urea.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
Purity
The purity of the protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
MAP3K12 binding inhibitory protein 1, MAPK upstream kinase-binding inhibitory protein, MUK-binding inhibitory protein, MBIP.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAATEL NRPSSGDRNL ERRCRPNLSR EVLYEIFRSL HTLVGQLDLR DDVVKITIDW NKLQSLSAFQ PALLFSALEQ HILYLQPFLA KLQSPIKEEN TTAVEEIGRT EMGNKNEVND KFSIGDLQEE EKHKESDLRD VKKTQIHFDP EVVQIKAGKA EIDRRISAFI ERKQAEINEN NVREFCNVID CNQENSCART DAIFTPYPGF KSHVKVSRVV NTYGPQTRPE GIPGSGHKPN SMLRDCGNQA VEERLQNIEA HLRLQTGGPV PRDIYQRIKK LEDKILELEG ISPEYFQSVS FSGKRRKVQP PQQNYSLAEL DEKISALKQA LLRKSREAES MATHHLP.
Product Science Overview
Structure and Function
Role in Disease
MBIP has been implicated in various diseases, including cancer. For instance, it has been shown to drive the metastasis of non-small cell lung cancer (NSCLC) by activating matrix metalloproteinases (MMPs) through the JNK-dependent pathway . This makes MBIP a potential target for therapeutic interventions in cancer treatment.
Recombinant MBIP
Recombinant MBIP is produced using Escherichia coli (E. coli) expression systems. The recombinant protein typically includes an N-terminal His-tag, which facilitates its purification and detection . The protein is denatured and purified to a high degree of purity, often exceeding 85% as determined by SDS-PAGE .
Applications
Recombinant MBIP is used in various research applications, including:
- Studying the JNK/SAPK signaling pathway: Researchers use recombinant MBIP to understand how this pathway is regulated and its role in cellular stress responses.
- Cancer research: Given its role in cancer metastasis, recombinant MBIP is used to study the mechanisms of cancer progression and to develop potential therapeutic strategies.
- Drug development: MBIP can be a target for developing drugs aimed at inhibiting its function, thereby controlling diseases associated with its dysregulation.
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