MBD3 Human

Methyl-CpG Binding Domain Protein 3 Human Recombinant

Cat. No.

BT22939

Source

E.coli.

Synonyms

Methyl-CpG Binding Domain Protein 3, Methyl-CpG-Binding Domain Protein 3, Methyl-CpG-Binding Protein MBD3.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 85% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MBD3 Human Recombinant produced in E. coli is a single polypeptide chain containing 314 amino acids (1-291) and having a molecular mass of 35.2 kDa. MBD3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

Methyl-CpG-binding domain protein 3 (MBD3) is part of the MBD family, known for their role as transcriptional repressors. This family, comprising MECP2, MBD1, MBD2, MBD3, and MBD4 in humans, is characterized by the presence of a methyl-CpG binding domain (MBD). However, MBD3 stands out as it lacks the ability to bind methylated DNA, unlike its counterparts. It functions as a subunit within the NuRD complex, a multisubunit entity involved in nucleosome remodeling and histone deacetylase activities. MBD3 exhibits significant sequence similarity to MBD2 (isoform 1) and mouse Mbd3, with 71% and 94% identity, respectively. It plays a crucial role in connecting metastasis-associated protein 2 (MTA2) to the core histone deacetylase complex.

Description

Recombinant MBD3 Human, produced in E. coli, is a single polypeptide chain with a molecular weight of 35.2 kDa. It consists of 314 amino acids, with the MBD3 sequence spanning residues 1-291, and includes a 23 amino acid His-tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.

Physical Appearance

A clear and colorless solution, sterilized by filtration.

Formulation

The MBD3 solution is provided at a concentration of 0.25mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.2M NaCl, 1mM DTT, 1mM EDTA, and 40% glycerol.

Stability

For short-term storage (up to 4 weeks), the solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein, such as HSA or BSA (0.1%), is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.

Purity

The purity of MBD3 is determined to be greater than 85% using SDS-PAGE analysis.

Synonyms

Methyl-CpG Binding Domain Protein 3, Methyl-CpG-Binding Domain Protein 3, Methyl-CpG-Binding Protein MBD3.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERKRWE CPALPQGWER EEVPRRSGLS AGHRDVFYYS PSGKKFRSKP QLARYLGGSM DLSTFDFRTG KMLMSKMNKS RQRVRYDSSN QVKGKPDLNT ALPVRQTASI FKQPVTKITN HPSNKVKSDP QKAVDQPRQL FWEKKLSGLN AFDIAEELVK TMDLPKGLQG VGPGCTDETL LSAIASALHT STMPITGQLS AAVEKNPGVW LNTTQPLCKA FMVTDEDIRK QEELVQQVRK RLEEALMADM LAHVEELARD GEAPLDKACA EDDDEEDEEE EEEEPDPDPE MEHV.

Product Science Overview

Introduction

Methyl-CpG Binding Domain Protein 3 (MBD3) is a member of the MBD protein family, which is characterized by the presence of a methyl-CpG binding domain. This family of proteins plays a crucial role in the regulation of gene expression through interactions with methylated DNA. MBD3, in particular, is a significant component of the nucleosome remodeling and histone deacetylase (NuRD) complex, which is involved in chromatin remodeling and transcriptional repression.

Gene and Protein Structure

The MBD3 gene is located on chromosome 19 in humans and encodes a protein that is approximately 291 amino acids in length. Unlike other members of the MBD family, MBD3 does not bind to methylated DNA by itself. Instead, it binds to unmethylated CpG dinucleotides to a lesser degree. The protein shares a high degree of similarity with MBD2, another member of the MBD family, with which it shares 71% and 94% identity in humans and mice, respectively.

Function and Mechanism

MBD3 is a critical component of the NuRD complex, which combines nucleosome remodeling and histone deacetylase activities. This complex plays a pivotal role in the regulation of gene expression by modifying chromatin structure, thereby influencing the accessibility of transcriptional machinery to DNA. MBD3 acts as a transcriptional repressor and is involved in gene silencing. It recruits histone deacetylases and DNA methyltransferases, which are essential for the maintenance of chromatin structure and gene repression.

Biological Significance

MBD3 is involved in various biological processes, including development, differentiation, and disease. It has been implicated in the regulation of genes associated with autism spectrum disorder and other developmental conditions. Additionally, MBD3 plays a role in the epithelial-mesenchymal transition (EMT), a process critical for cancer metastasis. Its aberrant expression has been observed in several human malignancies, highlighting its importance in cancer biology.

Recombinant MBD3

Recombinant MBD3 is produced using recombinant DNA technology, which involves the insertion of the MBD3 gene into an expression vector, followed by the expression of the protein in a suitable host system, such as E. coli or mammalian cells. The recombinant protein is then purified using various chromatographic techniques to obtain a highly pure and functional protein. Recombinant MBD3 is used in research to study its function, interactions, and role in various biological processes.

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MBD3 Human

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