MAPKSP1 antibody
MAPBP, MP1, MAPK Scaffold Protein 1, Interacting Protein 1, MEK binding partner 1, Mitogen-Activated Protein Kinase kinase 1.
Description
Product Specs
MAPBP, MP1, MAPK Scaffold Protein 1, Interacting Protein 1, MEK binding partner 1, Mitogen-Activated Protein Kinase kinase 1.
MAPKSP1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT1F10AT.
Anti-human MAPKSP1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAPKSP1 protein 1-124 amino acids purified from E. coli.
Mouse IgG2b heavy chain and k light chain.
Product Science Overview
Mitogen-Activated Protein Kinase Scaffold Protein 1 (MAPKSP1), also known as LAMTOR3, MAP2K1IP1, or Ragulator Complex Protein LAMTOR3, is a scaffold protein that plays a crucial role in the extracellular signal-regulated kinase (ERK) cascade. This protein is essential for the proper functioning of the MAPK signaling pathway, which regulates various cellular processes, including proliferation, differentiation, and survival.
MAPKSP1 is a small protein with a molecular weight of approximately 14 kDa. It is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein. The primary function of MAPKSP1 is to facilitate the interaction between MAP kinase kinase (MAP2K1/MEK1) and MAP kinases (MAPK3/ERK1 and MAPK1/ERK2). By bringing these kinases into close proximity, MAPKSP1 enhances the efficiency of the phosphorylation cascade that activates the ERK signaling pathway .
The MAPK signaling pathway is a fundamental pathway in cell biology, and its alteration can lead to various human cancers and developmental disorders. The Ras/RAF/MEK/ERK (MAPK) signaling pathway is one of the best-defined pathways in cancer biology. Hyperactivation of this pathway is responsible for over 40% of human cancer cases . MAPKSP1, as a scaffold protein, plays a critical role in ensuring the proper activation and regulation of this pathway.
The mouse anti-human MAPKSP1 monoclonal antibody is a laboratory tool used to study the MAPKSP1 protein. This antibody is highly specific and recognizes human MAPKSP1. It is typically used in various applications, including ELISA (EIA) and Western Blot (WB), to detect the presence and quantify the levels of MAPKSP1 in biological samples .
The antibody is purified by Protein A affinity chromatography and is supplied as a liquid in PBS, pH 7.2, without any preservatives. It is labeled with horseradish peroxidase (HRP) for detection purposes. The immunogen used to generate this antibody is a partial recombinant protein corresponding to amino acids 1-88 of MAPKSP1, tagged with GST .
The mouse anti-human MAPKSP1 monoclonal antibody is used extensively in research to study the role of MAPKSP1 in various cellular processes and diseases. It is particularly useful in cancer research, where the MAPK signaling pathway is often dysregulated. By using this antibody, researchers can gain insights into the mechanisms of MAPKSP1 function and its involvement in disease progression .
