MAPKAPK3 Human

Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 Human Recombinant

Cat. No.

BT11232

Source

E.coli.

Synonyms

3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3,MAPKAPK-3, MK-3,MAPKAPK3.

Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95% as determined by SDS-PAGE.

Usage

THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

MAPKAPK3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 405 amino acids (1-382 a.a) and having a molecular mass of 45.4kDa. MAPKAPK3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

MAPKAPK3 (MAP kinase-activated protein kinase 3) plays a role in inflammatory responses by regulating the production of TNF (tumor necrosis factor) and IL6 at the post-transcriptional level. It achieves this by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, which influences the stability and translation of TNF and IL6 mRNAs. The phosphorylation of TTP/ZFP36, a key post-transcriptional regulator of TNF, enhances its interaction with 14-3-3 proteins and diminishes its affinity for ARE mRNA. This, in turn, impedes the degradation of transcripts containing AREs. MAPKAPK3 becomes activated in response to growth factors and cellular stress.

Description

Recombinant Human MAPKAPK3, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 405 amino acids (amino acids 1-382). It has a molecular weight of 45.4 kDa. The MAPKAPK3 protein is fused to a 23 amino acid His-tag at its N-terminus and is purified using proprietary chromatographic techniques.

Physical Appearance

A clear, colorless solution that has been sterilized by filtration.

Formulation

The MAPKAPK3 protein solution has a concentration of 1 mg/ml and is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 20% glycerol, and 1 mM DTT.

Stability

For short-term storage (up to 4 weeks), the protein should be kept at 4°C. For extended storage, it is recommended to freeze the protein at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein such as HSA or BSA (0.1%). It's important to avoid repeated freeze-thaw cycles.

Purity

The purity of the MAPKAPK3 protein is greater than 95% as determined by SDS-PAGE analysis.

Synonyms

3PK, MAPKAP-K3, MAPKAP3, MAPKAPK-3, MK-3, MAP kinase-activated protein kinase 3, MAPK-activated protein kinase 3, MAPKAP kinase 3,MAPKAPK-3, MK-3,MAPKAPK3.

Source

E.coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMDGETAE EQGGPVPPPV APGGPGLGGA PGGRREPKKY AVTDDYQLSK QVLGLGVNGK VLECFHRRTG QKCALKLLYD SPKARQEVDH HWQASGGPHI VCILDVYENM HHGKRCLLII MECMEGGELF SRIQERGDQA FTEREAAEIM RDIGTAIQFL HSHNIAHRDV KPENLLYTSK EKDAVLKLTD FGFAKETTQN ALQTPCYTPY YVAPEVLGPE KYDKSCDMWS LGVIMYILLC GFPPFYSNTG QAISPGMKRR IRLGQYGFPN PEWSEVSEDA KQLIRLLLKT DPTERLTITQ FMNHPWINQS MVVPQTPLHT ARVLQEDKDH WDEVKEEMTS ALATMRVDYD QVKIKDLKTS NNRLLNKRRK KQAGSSSASQ GCNNQ.

Product Science Overview

Introduction

Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3), also known as MK3, is a member of the Serine/Threonine protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals.

Structure and Function

MAPKAPK3 is encoded by the MAPKAPK3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK.

Biological Role

MAPKAPK3 plays a crucial role in various cellular processes, including proliferation, differentiation, and cell cycle progression. It is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. This kinase is involved in the regulation of gene expression, cellular growth, and apoptosis.

Clinical Significance

Mutations or dysregulation of MAPKAPK3 have been associated with various diseases, including cancer and inflammatory disorders. The protein’s role in the MAP kinase signaling pathway makes it a potential target for therapeutic interventions in diseases where this pathway is dysregulated.

Research and Applications

Human recombinant MAPKAPK3 is used in research to study its function and role in various signaling pathways. It is also used in drug discovery and development to identify potential inhibitors that can modulate its activity. The recombinant form of this protein allows for detailed biochemical and structural studies, providing insights into its mechanism of action and potential therapeutic applications.

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MAPKAPK3 Human

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