Recombinant human MAK16 protein is typically expressed in E. coli and purified using conventional chromatography techniques . The protein is often tagged with a His-tag at the N-terminus to facilitate purification and detection . The amino acid sequence of the recombinant MAK16 protein includes the following residues:
MGSSHHHHHH SSGLVPRGSH MGSMQSDDVI WDTLGNKQFC SFKIRTKTQS FCRNEYSLTG LCNRSSCPLA NSQYATIKEE KGQCYLYMKV IERAAFPRRL WERVRLSKNY EKALEQIDEN LIYWPRFIRH KCKQRFTKIT QYLIRIRKLT LKRQRKLVPL SKKVERREKR REEKALIAAQ LDNAIEKELL ERLKQDTYGD IYNFPIHAFD KALEQQEAES DSSDTEEKDD DDDDEEDVGK REFVEDGEVD ESDISDFEDM DKLDASSDED QDGKSSSEEE EEKALSAKHK GKMPLRGPLQ RKRAYVEIEY EQETEPVAKA KTT
The predicted molecular mass of the recombinant MAK16 protein is approximately 37.8 kDa . However, the observed molecular weight may vary due to post-translational modifications and other experimental factors .
MAK16, also known as RNA-binding motif protein 13 (RBM13), is a member of the MAK16 family of proteins . Despite its name, RBM13 has not been shown to be an RNA-binding protein, and its exact function remains unknown . The MAK16 family is involved in the maintenance of killer protein 16, which plays a role in various cellular processes .
Recombinant human MAK16 protein is used in various research applications, including:
The recombinant human MAK16 protein is typically formulated in a buffer containing PBS (pH 7.4), 30% glycerol, and 1 mM DTT . It is shipped with polar packs and should be stored at 4°C for short-term use or aliquoted and stored at -20°C for long-term use . It is important to avoid repeated freeze-thaw cycles to maintain the protein’s stability and activity .