MAFG Human

V-maf Musculoaponeurotic Fibrosarcoma Oncogene G Human Recombinant
Cat. No.
BT26136
Source
Escherichia Coli.
Synonyms
Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG, hMAF.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAFG Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-162) and having a molecular mass of 20kDa.
MAFG is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The transcription factor MafG (MAFG) belongs to the Maf family. Lacking a putative transactivation domain, small Mafs like MAFG act as transcriptional repressors when forming dimers with themselves. However, they can function as transcriptional activators by dimerizing with other larger basic-zipper proteins, directing them to specific DNA binding sites. Notably, small Maf proteins can heterodimerize with Fos, potentially acting as competitive repressors of the NF-E2 transcription factor.
Description
Recombinant human MAFG protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 182 amino acids (residues 1-162). This protein has a molecular weight of 20 kDa. For purification and detection purposes, a 20 amino acid His-tag is fused to the N-terminus of the MAFG protein. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The MAFG protein solution is supplied at a concentration of 0.25 mg/ml. It is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 40% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the MAFG protein should be kept at 4°C. For extended storage, it is recommended to store the protein at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the MAFG protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
Transcription factor MafG, V-maf musculoaponeurotic fibrosarcoma oncogene homolog G, hMAF, MAFG, hMAF.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV ITIVKSKTDA RS.

Product Science Overview

Structure and Function

MAFG is a small basic leucine zipper (bZIP) transcription factor that forms homodimers or heterodimers with other bZIP proteins. These dimers bind to specific DNA sequences, regulating the expression of target genes involved in oxidative stress response, detoxification, and cellular differentiation . MAFG, along with other MAF proteins, is known to interact with the antioxidant response element (ARE) in the promoter regions of genes encoding detoxifying enzymes and antioxidant proteins.

Role in Disease

MAFG has been implicated in various diseases, including cancer and neurodegenerative disorders. Its overexpression has been observed in certain types of cancer, where it may contribute to tumor progression by promoting cell survival and proliferation . Additionally, MAFG has been linked to the regulation of genes involved in the oxidative stress response, which is a critical factor in the pathogenesis of neurodegenerative diseases such as Alzheimer’s and Parkinson’s .

Human Recombinant MAFG

Human recombinant MAFG is a synthetically produced version of the protein, often used in research to study its function and potential therapeutic applications. One notable application of human recombinant MAFG is in the reprogramming of human pancreatic duct-derived cells (HDDCs) into insulin-secreting cells. This innovative approach involves the use of synthetic modified mRNA encoding MAFG, which is transfected into HDDCs to induce their differentiation into β-like cells capable of producing insulin .

Research and Therapeutic Potential

The ability to reprogram HDDCs into insulin-secreting cells using human recombinant MAFG holds significant promise for the treatment of type 1 diabetes. This approach offers a potential alternative to β-cell replacement therapy, which aims to restore β-cell mass and glucose homeostasis in diabetic patients . The use of non-integrative RNA-based reprogramming techniques ensures that the genetic modifications are transient and do not integrate into the host genome, reducing the risk of adverse effects .

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