MAFF Human

V-maf Musculoaponeurotic Fibrosarcoma Oncogene F Human Recombinant
Cat. No.
BT26054
Source
E.coli.
Synonyms
hMafF, U-MAF, Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

MAFF Human Recombinant produced in E. coli is a single polypeptide chain containing 187 amino acids (1-164) and having a molecular mass of 20.1kDa. MAFF is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The V-maf Musculoaponeurotic Fibrosarcoma Oncogene F (MAFF), primarily found in the uterine muscle (myometrium) and kidneys, is a crucial protein belonging to the basic leucine zipper (bZIP) transcription factor family. Unlike many transcription factors, MAFF lacks a transactivation domain, implying its role in regulating gene expression through interactions with other proteins. MAFF exhibits binding affinity to the US-2 DNA element and is thought to form heterodimers with other leucine zipper-containing proteins. This interaction is particularly important during pregnancy, where it enhances the expression of the OTR gene. Interestingly, MAFF can also form homodimers; however, due to the absence of a transactivation domain, these homodimers function as transcriptional repressors.
Description
Recombinant MAFF protein, produced in E. coli, is a single polypeptide chain with a molecular weight of 20.1kDa. It consists of 187 amino acids, with the first 164 amino acids representing the MAFF protein and a 23 amino acid His-tag fused at the N-terminus for purification purposes. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The MAFF solution is provided at a concentration of 1mg/ml in a buffer consisting of 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (2-4 weeks), the MAFF solution can be stored at 4°C. For long-term storage, it is recommended to store the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage to maintain protein stability. Avoid repeated freezing and thawing cycles.
Purity
The purity of the MAFF protein is greater than 85% as determined by SDS-PAGE analysis.
Synonyms
hMafF, U-MAF, Transcription factor MafF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, MAFF.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMSVDPLS SKALKIKREL SENTPHLSDE ALMGLSVREL NRHLRGLSAE EVTRLKQRRR TLKNRGYAAS CRVKRVCQKE ELQKQKSELE REVDKLAREN AAMRLELDAL RGKCEALQGF ARSVAAARGP ATLVAPASVI TIVKSTPGSG SGPAHGPDPA HGPASCS.

Product Science Overview

Structure and Function

The MAFF protein contains a bZIP domain, which is essential for DNA binding and dimerization. This domain allows MAFF to bind to specific DNA sequences and regulate the transcription of target genes. The protein is involved in the regulation of oxidative stress responses and has been implicated in various physiological and pathological processes, including cancer, diabetes, and neurodegenerative diseases .

Role in Disease

MAFF has been associated with several diseases, including fibrosarcoma, a type of cancer that arises from fibrous connective tissue. The overexpression of MAFF has been observed in certain cancer types, suggesting its potential role in tumorigenesis. Additionally, MAFF is involved in the regulation of insulin secretion and glucose homeostasis, making it a potential target for diabetes research .

Research and Applications

Recent studies have explored the use of synthetic modified mRNA to overexpress MAFF in human pancreatic duct-derived cells (HDDCs). This approach has shown promise in reprogramming these cells into insulin-secreting cells, which could be used for β-cell replacement therapy in patients with type 1 diabetes. The overexpression of MAFF in HDDCs has been shown to induce β-cell differentiation and insulin secretion in response to glucose stimulation, providing a potential therapeutic strategy for diabetes .

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THE BIOTEK
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