MAD2L1BP Human
MAD2L1 Binding Protein Human Recombinant
Cat. No.
BT4643
Source
Escherichia Coli.
Synonyms
MAD2L1-binding protein, Caught by MAD2 protein, MAD2L1BP, CMT2, KIAA0110, RP1-261G23.6.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
MAD2L1BP Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 298 amino acids (1-274 a.a) and having a molecular mass of 33.6kDa (Molecular weight on SDS-PAGE will appear higher).
MAD2L1BP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
MAD2L1BP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
MAD2L1-binding protein (MAD2L1BP) belongs to the MAD2L1BP family and plays a crucial role in cell cycle regulation. This protein binds to MAD2, a key component of the spindle checkpoint that ensures accurate chromosome segregation during cell division. MAD2L1BP regulates the spindle checkpoint by controlling MAD2's activity. It promotes the release of MAD2 from its complex with CDC20, thereby allowing mitosis to progress to anaphase. Throughout the cell cycle, MAD2L1BP levels rise and stay elevated until late mitosis when they decline.
Description
This product consists of the recombinant human MAD2L1BP protein, expressed in E. coli and purified to a high degree. The protein is not glycosylated and comprises 298 amino acids, including a 24-amino acid His-tag at the N-terminus. With a molecular weight of 33.6 kDa, it may appear larger on SDS-PAGE due to the His-tag. The protein is purified using advanced chromatographic methods to ensure high purity.
Physical Appearance
The product is a clear solution that has been sterilized by filtration.
Formulation
This product is supplied in a solution containing 0.5mg/ml MAD2L1BP protein, 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, and 100mM NaCl.
Stability
For short-term storage (up to four weeks), keep the product at 4°C. For extended storage, freeze the product at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is suggested for long-term storage. It's important to avoid repeatedly freezing and thawing the product.
Purity
The purity of this product is over 90%, as determined by SDS-PAGE analysis.
Synonyms
MAD2L1-binding protein, Caught by MAD2 protein, MAD2L1BP, CMT2, KIAA0110, RP1-261G23.6.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAAPEA EVLSSAAVPD LEWYEKSEET HASQIELLET SSTQEPLNAS EAFCPRDCMV PVVFPGPVSQ EGCCQFTCEL LKHIMYQRQQ LPLPYEQLKH FYRKPSPQAE EMLKKKPRAT TEVSSRKCQQ ALAELESVLS HLEDFFARTL VPRVLILLGG
NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE.
NALSPKEFYE LDLSLLAPYS VDQSLSTAAC LRRLFRAIFM ADAFSELQAP PLMGTVVMAQ GHRNCGEDWF RPKLNYRVPS RGHKLTVTLS CGRPSIRTTA WEDYIWFQAP VTFKGFRE.
Product Science Overview
Function and Mechanism
MAD2L1BP is involved in the regulation of the spindle checkpoint during mitosis. The spindle checkpoint ensures that all chromosomes are properly attached to the spindle microtubules before the cell proceeds to anaphase, thus preventing chromosome missegregation and aneuploidy . MAD2L1BP binds to MAD2L1, a key component of the spindle checkpoint, and helps in silencing the checkpoint to allow the cell to proceed through anaphase .
Expression and Localization
Recombinant MAD2L1BP
Recombinant human MAD2L1BP protein is produced using an expression system in Escherichia coli (E. coli). The recombinant protein is typically fused to a His-tag at the N-terminus to facilitate purification . The protein is purified using conventional chromatography techniques and is available in a liquid formulation containing Tris-HCl buffer, DTT, glycerol, and NaCl .
Applications
Storage and Handling
References
- Recombinant human MAD2L1BP protein - ibl-america.com
- MAD2L1BP gene information - The Human Protein Atlas
- MAD2L1BP Gene - MAD2L1 Binding Protein - GeneCards
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