M. Tuberculosis RV1681 Antibody

Mycobacterium Tuberculosis RV1681, Polyclonal Rabbit Antibody
Cat. No.
BT4734
Source
Synonyms
Appearance
Sterile Filtered clear colorless solution.
Purity
Pure Rabbit IgG, >95% pure as determined by 12% PAGE (coomassie staining).
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The polyclonal antibody to Mycobacterium Tuberculosis RV1681 was collected from the rabbit igG and immunized with recombinant  M. tuberculosis t  RV-1681.

Product Specs

Introduction
Mycobacterium tuberculosis, a bacterium belonging to the Mycobacteriaceae family, is a significant infectious pathogen responsible for the majority of tuberculosis cases. It ranks as the second leading cause of death globally among infectious diseases. Diagnosing M. tuberculosis infection traditionally relies on culturing the bacteria from patient specimens, a method considered the gold standard but often expensive and time-intensive. RV-1681, a protein secreted by M. tuberculosis within infected organs, enters the bloodstream and is eventually filtered into the urine by the kidneys.
RV-1681 is viewed as a promising marker for M. tuberculosis detection, potentially enabling a simple, cost-effective, and rapid urine test to screen large populations for the infection.
Description
Polyclonal antibodies against Mycobacterium Tuberculosis RV1681 were generated. The antibodies were isolated from the immunoglobulin G (igG) fraction of serum from rabbits immunized with a recombinant fragment of the M. tuberculosis RV-1681 protein.
Purity
The antibody preparation consists of pure Rabbit IgG that is greater than 95% pure. Purity was assessed by 12% polyacrylamide gel electrophoresis followed by Coomassie blue staining.
Physical Appearance
The antibody solution is sterile-filtered and appears as a clear, colorless liquid.
Formulation
The antibodies are supplied in a buffered solution consisting of Glycine and Tris, at pH 7.5.
Stability
For short-term storage (2-4 weeks), the antibody solution should be kept at 4°C. For extended storage, it is recommended to store the solution frozen at -20°C.
To enhance long-term stability during storage, the addition of a carrier protein such as albumin (0.1% HSA or BSA) is advisable.
Repeated cycles of freezing and thawing should be avoided.
Purification Method

Purified by affinity chromatographic technique.

Product Science Overview

Introduction

Mycobacterium tuberculosis (M. tuberculosis) is the bacterium responsible for tuberculosis (TB), a serious infectious disease that primarily affects the lungs but can also impact other parts of the body. The identification and study of specific proteins and antibodies related to M. tuberculosis are crucial for understanding the pathogenesis of TB and developing diagnostic and therapeutic tools.

RV1681 Protein

The RV1681 protein is one of the many proteins encoded by the M. tuberculosis genome. Proteins like RV1681 play various roles in the bacterium’s survival, virulence, and interaction with the host’s immune system. Studying these proteins helps researchers understand the mechanisms M. tuberculosis uses to evade the immune system and persist in the host.

Polyclonal Rabbit Antibody

Polyclonal antibodies are a mixture of antibodies produced by different B cell clones in the body. These antibodies recognize and bind to multiple epitopes on a single antigen. Polyclonal rabbit antibodies are commonly used in research due to their ability to recognize multiple epitopes, which can increase the sensitivity of detection methods.

Production of Polyclonal Rabbit Antibody Against RV1681

To produce a polyclonal rabbit antibody against the RV1681 protein, the following steps are typically followed:

  1. Antigen Preparation: The RV1681 protein is purified and prepared as an antigen.
  2. Immunization: Rabbits are immunized with the RV1681 antigen. The immune system of the rabbits responds by producing antibodies against the antigen.
  3. Collection: Blood is collected from the immunized rabbits, and the serum, which contains the polyclonal antibodies, is separated.
  4. Purification: The polyclonal antibodies are purified from the serum for use in various applications.
Applications

Polyclonal rabbit antibodies against RV1681 can be used in several research applications, including:

  • Western Blotting: To detect the presence of RV1681 protein in samples.
  • Immunohistochemistry (IHC): To visualize the localization of RV1681 protein in tissue sections.
  • Enzyme-Linked Immunosorbent Assay (ELISA): To quantify the amount of RV1681 protein in samples.
  • Flow Cytometry: To analyze the expression of RV1681 protein on the surface of cells.

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