LZTFL1 Human
Leucine Zipper Transcription Factor-Like 1 Human Recombinant
Cat. No.
BT22146
Source
Escherichia Coli.
Synonyms
Leucine Zipper Transcription Factor-Like 1, BBS17, Leucine
Zipper Transcription Factor-Like Protein 1, Leucine zipper transcription
factor-like protein 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
LZTFL1 Human Recombinant produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 322 amino acids (1-299 a.a) and having a molecular
mass of 37.0kDa.
LZTFL1 is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Introduction
LZTFL1, short for Leucine Zipper Transcription Factor-Like 1, is a protein found throughout the body. It resides in the cell's cytoplasm and plays a role in cellular function by interacting with Bardet-Biedl Syndrome (BBS) proteins. This interaction is important for regulating the movement of proteins to the ciliary membrane, a hair-like structure on cells. Mutations in the LZTFL1 gene can lead to a form of Bardet-Biedl Syndrome, a disorder characterized by various symptoms including extra fingers or toes, obesity, intellectual disabilities, hormonal imbalances, and kidney problems. Furthermore, LZTFL1 may act as a tumor suppressor, potentially through its interaction with E-cadherin and the actin cytoskeleton, thereby influencing the transition of epithelial cells into mesenchymal cells.
Description
Recombinant human LZTFL1, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 322 amino acids (with amino acids 1 to 299 present) and possessing a molecular weight of 37.0 kDa. A 23-amino acid His-tag is fused to the N-terminus of the LZTFL1 protein. Purification is achieved using proprietary chromatographic methods.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The provided LZTFL1 protein solution has a concentration of 1 mg/ml and is prepared in a buffer solution consisting of phosphate-buffered saline (PBS) at a pH of 7.4, 10% glycerol, and 1 mM dithiothreitol (DTT).
Stability
For short-term storage (up to 2-4 weeks), the solution should be kept refrigerated at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To further enhance stability during long-term storage, consider adding a carrier protein such as albumin (0.1% HSA or BSA). Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the LZTFL1 protein is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Leucine Zipper Transcription Factor-Like 1, BBS17, Leucine
Zipper Transcription Factor-Like Protein 1, Leucine zipper transcription
factor-like protein 1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAELGLN EHHQNEVINY MRFARSKRGL RLKTVDSCFQ DLKESRLVED
TFTIDEVSEV LNGLQAVVHS EVESELINTA YTNVLLLRQL FAQAEKWYLK LQTDISELEN RELLEQVAEF
EKAEITSSNK KPILDVTKPK LAPLNEGGTA ELLNKEILRL QEENEKLKSR LKTIEIQATN ALDEKSKLEK
ALQDLQLDQG NQKDFIKAQD LSNLENTVAA LKSEFQKTLN DKTENQKSLE ENLATAKHDL LRVQEQLHMA
EKELEKKFQQ TAAYRNMKEI LTKKNDQIKD LRKRLAQYEP ED.
Product Science Overview
Gene and Protein Structure
LZTFL1 is located on chromosome 3p21.3, a region known for its high density of tumor suppressor genes . The gene encodes a protein that contains a leucine zipper domain, which is a common motif involved in protein-protein interactions. The leucine zipper domain allows LZTFL1 to form dimers and interact with other proteins, facilitating its role in various cellular processes .
Biological Functions
LZTFL1 is primarily localized in the cytoplasm and cilia of cells . It plays a crucial role in several cellular functions, including:
- Ciliary Trafficking: LZTFL1 is involved in the trafficking of proteins to and from the cilia, which are hair-like structures on the cell surface that play a key role in cell signaling and sensory perception .
- Immune Synapse Formation: LZTFL1 is induced by all-trans retinoic acid in activated T cells and associates with the immune synapse, suggesting a role in immune response regulation .
- Tumor Suppression: LZTFL1 acts as a tumor suppressor by negatively regulating the hedgehog signaling pathway, which is involved in cell growth and differentiation .
Clinical Significance
Research and Applications
Recent studies have highlighted the role of LZTFL1 in intracellular transport and protein trafficking. For instance, LZTFL1 has been shown to bind to adaptor protein complexes AP-1 and AP-2, which are involved in the trafficking of the transferrin receptor 1 (TfR1) . Knockout studies in mice have demonstrated that the absence of LZTFL1 leads to abnormal distribution of these adaptor proteins, further underscoring its role in cellular transport mechanisms .
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