LZIC Human

Leucine Zipper And CTNNBIP1 Domain Containing Human Recombinant
Cat. No.
BT22049
Source
Escherichia Coli.
Synonyms
WAP Four-Disulfide Core Domain 12, Putative Protease Inhibitor WAP12, Whey Acidic Protein 2, Chromosome 20 Open Reading Frame 122, Protease Inhibitor WAP2, Single WAP Motif Protein 2, WAP Four-Disulfide Core Domain Protein 12, dJ211D12.4, C20orf122, SWAM2, WAP2.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 85% as determined by SDS-PAGE.
Usage
THE BioTeks products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LZIC Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 111 amino acids (24-111) and having a molecular mass of 12.1 kDa. LZIC is fused to a 23 amino acid His-tag at N-terminus.

Product Specs

Introduction
LZIC, a member of the CTNNBIP1 family, is ubiquitously expressed, with highest levels found in the kidneys. Its expression is elevated in various gastric cancer cases. LZIC does not interact with CTNNB1.
Description
Recombinant human LZIC, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 111 amino acids (residues 24-111) with a molecular weight of 12.1 kDa. A 23 amino acid His-tag is fused to the N-terminus of LZIC.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The LZIC solution (1 mg/ml) is formulated in 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Repeated freezing and thawing should be avoided.
Purity
Purity exceeds 85% as determined by SDS-PAGE analysis.
Synonyms
WAP Four-Disulfide Core Domain 12, Putative Protease Inhibitor WAP12, Whey Acidic Protein 2, Chromosome 20 Open Reading Frame 122, Protease Inhibitor WAP2, Single WAP Motif Protein 2, WAP Four-Disulfide Core Domain Protein 12, dJ211D12.4, C20orf122, SWAM2, WAP2.
Source
Escherichia Coli.
Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMASRGKT ETSKLKQNLE EQLDRLMQQL QDLEECREEL DTDEYEETKK ETLEQLSEFN DSLKKIMSGN MTLVDELSGM QLAIQAAISQ AFKTPEVIRL FAKKQPGQLR TRLAEMDRDL MVGKLERDLY TQQKVEILTA LRKLGEKLTA DDEAFLSANA GAILSQFEKV STDLGSGDKI LALASFEVEK TKK.

Product Science Overview

Introduction

The Leucine Zipper And CTNNBIP1 Domain Containing (LZIC) protein is a human recombinant protein encoded by the LZIC gene. This protein is significant due to its involvement in various cellular processes, particularly those related to beta-catenin binding and response to ionizing radiation .

Gene and Protein Structure

The LZIC gene is located on chromosome 1p36.22 and encodes a protein consisting of 190 amino acids . The protein contains two primary domains:

  1. Leucine Zipper Domain: This domain is characterized by the presence of leucine residues at every seventh position, forming a coiled-coil structure that facilitates protein-protein interactions.
  2. CTNNBIP1 (ICAT-like) Domain: This domain shares 38% amino acid identity with the ICAT gene and is involved in beta-catenin binding .
Function and Significance

The LZIC protein is predicted to enable beta-catenin binding activity, which is crucial for various signaling pathways, including the Wnt signaling pathway . This pathway plays a vital role in cell proliferation, differentiation, and migration. Additionally, LZIC is involved in the cellular response to ionizing radiation, suggesting its potential role in DNA damage repair mechanisms .

Clinical Relevance

Mutations or dysregulation of the LZIC gene have been associated with certain diseases, including Corneal Dystrophy, Thiel-Behnke Type . Understanding the function and regulation of LZIC can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.

Recombinant Protein Production

The human recombinant LZIC protein is produced using HEK293T cells, a widely used human cell line for protein expression . The recombinant protein is captured through an anti-DDK affinity column followed by conventional chromatography steps to ensure high purity and stability . The protein is available in various quantities and is used in research to study its function and interactions.

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