LYN Human
v-yes-1 Yamaguchi Sarcoma Viral Related Oncogene Human Recombinant
Cat. No.
BT21983
Source
Escherichia Coli.
Synonyms
Tyrosine-protein kinase Lyn, Lck/Yes-related novel
protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene
homolog, p53Lyn, p56Lyn, JTK8, EC 2.7.10, LYN Proto-Oncogene, Src Family
Tyrosine Kinase, V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog,
Lck/Yes-Related Novel Protein Tyrosine Kinase, EC 2.7.10.2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
LYN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 535 amino acids (1-512) and having a molecular mass of 61.0kDa. LYN is fused to a 23 amino acid His-tag at N-terminus.
Product Specs
Introduction
Tyrosine-protein kinase Lyn (LYN) is a member of the src family of non-receptor protein tyrosine kinases. Primarily found in haematopoietic tissues, LYN plays a role in activating the stress-activated protein kinase (SAPK) pathway in response to DNA damage. It does not, however, activate ERK or p38 MAPK pathways. LYN's stimulation of SAPK involves a mechanism dependent on MKK7 and MEKK1. This LYN - MEKK1 - MKK7 - SAPK pathway is significant in triggering apoptosis as a response to genotoxic agents.
Description
Recombinant human LYN protein, produced in E.coli, is a single, non-glycosylated polypeptide chain. It consists of 535 amino acids (residues 1-512), with a molecular weight of 61.0kDa. The LYN protein has a 23 amino acid His-tag fused to its N-terminus.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The LYN solution is provided at a concentration of 1mg/ml. It is formulated in a buffer consisting of 20mM Tris-HCl (pH 8.0), 0.4M urea, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep refrigerated at 4°C. For extended periods, store frozen at -20°C. To ensure stability during long-term storage, adding a carrier protein like 0.1% HSA or BSA is advised. Repeated freezing and thawing should be avoided.
Purity
Analysis by SDS-PAGE indicates a purity greater than 85.0%.
Synonyms
Tyrosine-protein kinase Lyn, Lck/Yes-related novel
protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene
homolog, p53Lyn, p56Lyn, JTK8, EC 2.7.10, LYN Proto-Oncogene, Src Family
Tyrosine Kinase, V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog,
Lck/Yes-Related Novel Protein Tyrosine Kinase, EC 2.7.10.2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH
SSGLVPRGSH MGSMGCIKSK
GKDSLSDDGV DLKTQPVRNT ERTIYVRDPT SNKQQRPVPE SQLLPGQRFQ TKDPEEQGDI VVALYPYDGI
HPDDLSFKKG EKMKVLEEHG EWWKAKSLLT KKEGFIPSNY VAKLNTLETE EWFFKDITRK DAERQLLAPG
NSAGAFLIRE SETLKGSFSL SVRDFDPVHG DVIKHYKIRS LDNGGYYISP RITFPCISDM IKHYQKQADG
LCRRLEKACI SPKPQKPWDK DAWEIPRESI KLVKRLGAGQ FGEVWMGYYN NSTKVAVKTL KPGTMSVQAF
LEEANLMKTL QHDKLVRLYA VVTREEPIYI ITEYMAKGSL LDFLKSDEGG KVLLPKLIDF SAQIAEGMAY
IERKNYIHRD LRAANVLVSE SLMCKIADFG LARVIEDNEY TAREGAKFPI KWTAPEAINF GCFTIKSDVW SFGILLYEIV
TYGKIPYPGR TNADVMTALS QGYRMPRVEN CPDELYDIMK MCWKEKAEER PTFDYLQSVL DDFYTATEGQ
YQQQP.
Product Science Overview
Introduction
The v-yes-1 Yamaguchi Sarcoma Viral Related Oncogene (YES1) is a non-receptor protein tyrosine kinase that plays a crucial role in various cellular processes, including cell growth, survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation . It is the cellular homolog of the Yamaguchi sarcoma virus oncogene and belongs to the src family of proteins .
Gene and Protein Structure
The YES1 gene is located on chromosome 18p11.32 and encodes a protein with a molecular mass of approximately 60.8 kDa . The protein consists of several important domains:
- SRC Homology-3 (SH3) Domain: Involved in protein-protein interactions.
- SRC Homology-2 (SH2) Domain: Binds to phosphorylated tyrosine residues.
- Tyrosine Kinase Domain: Responsible for its kinase activity .
The YES1 protein shares a high degree of homology with the v-src gene product of the Rous sarcoma virus .
Expression Patterns and Tissue Distribution
Biological Functions
YES1 is involved in several critical cellular functions:
- Cell Growth and Survival: It regulates cell growth and survival through its kinase activity.
- Apoptosis: YES1 plays a role in programmed cell death.
- Cell-Cell Adhesion and Cytoskeleton Remodeling: It is involved in maintaining cell structure and interactions .
- Differentiation: YES1 influences the differentiation of cells into specific types .
Role in Immune Responses and Pathogen Recognition
YES1 is implicated in immune responses, particularly in the activation of transcriptional regulators that control tissue growth and regeneration . It is activated upon receptor engagement to phosphorylate YAP and induce its stabilization and nuclear translocation, promoting healing and maintaining barrier function .
Modes of Action and Mechanisms with Other Molecules
Binding Partners and Downstream Signaling Cascades
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