Lungkine Mouse

The gene encoding mouse Lungkine is located on chromosome 5 . The cDNA of mouse Lungkine encodes a protein consisting of 167 amino acids . This includes a 25 amino acid signal peptide and a 142 amino acid mature protein . The mature protein has an exceptionally long C-terminal tail that extends beyond the typical chemokine fold .

Lungkine is primarily expressed in the adult and fetal lung . Its expression is up-regulated under inflammatory conditions, indicating its role in immune responses . Lungkine is secreted into the bronchoalveolar space and is involved in lung-specific neutrophil trafficking . Studies using Lungkine knockout mice have shown that Lungkine is a critical mediator of neutrophil migration from the lung parenchyma into the airspace .

Additionally, Lungkine is chemotactic for bone marrow progenitor cells and modulates hematopoietic cell differentiation . This suggests that Lungkine not only plays a role in immune responses but also in the regulation of hematopoiesis.

Recombinant mouse Lungkine is typically produced in Escherichia coli (E. coli) . The recombinant protein is a single, non-glycosylated polypeptide chain containing 142 amino acids and has a molecular mass of approximately 16.4 kDa . The protein is purified using proprietary chromatographic techniques to ensure high purity .

Recombinant Lungkine is used in various research applications, particularly in studies related to inflammation, immune response, and hematopoiesis. It is also used to investigate the mechanisms of neutrophil trafficking and the role of chemokines in lung-specific immune responses.

The lyophilized recombinant Lungkine protein is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage . Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for long-term use . To prevent degradation, it is recommended to add a carrier protein such as human or bovine serum albumin .