Introduction
LSM5, also known as LSM5 Homolog or U6 Small Nuclear RNA Associated, is a member of the Sm protein family. This family is found in various organisms and is characterized by the presence of the Sm sequence motif. This motif consists of two regions separated by a variable-length linker that forms a loop. Sm-like proteins are believed to assemble into a stable heteromer found in tri-snRNP particles, which play a crucial role in pre-mRNA splicing. LSM5 has been linked to diseases like spinal muscular atrophy and muscular atrophy.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml in a solution of phosphate-buffered saline (PBS) at pH 7.4, with 10% glycerol and 0.02% sodium azide added as preservatives.
Storage Procedures
For short-term storage (up to 1 month), the antibody should be kept at 4°C. For longer storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
The LSM5 antibody has undergone rigorous testing in various applications, including ELISA, Western blot analysis, flow cytometry, and immunocytochemistry/immunofluorescence (ICC/IF), to ensure its specificity and reactivity. However, it is essential to optimize the antibody concentration for each specific application to achieve optimal results.
Synonyms
LSM5 homolog, U6 small nuclear RNA associated (S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.
Purification Method
LSM5 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Immunogen
Anti-human LSM5 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LSM5 protein 1-91 amino acids purified from E. coli.
Ig Subclass
Mouse IgG2a heavy chain and k light chain.
