LRRC59 Human

Leucine Rich Repeat Containing 59 Human Recombinant

Cat. No.

BT21396

Source

Escherichia Coli.

Synonyms

Leucine Rich Repeat Containing 59, Ribosome-Binding Protein P34, p34, Leucine-Rich Repeat-Containing Protein 59.

Appearance

Sterile Filtered clear solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Usage

Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Description

LRRC59 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 267 amino acids (1-244) and having a molecular mass of 30.3kDa.
LRRC59 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction

LRRC59 facilitates the entry of external FGF1 into the cell nucleus by binding it to the nuclear import machinery and guiding it through nuclear pores. This process is crucial for FGF1 to reach the nucleus, but not required for FGF2.

Description

Recombinant LRRC59 protein, manufactured in E. coli bacteria, is a single chain of 267 amino acids (specifically, amino acids 1 to 244) that lacks glycosylation. It has a molecular weight of 30.3 kDa. The protein includes a 23 amino acid His-tag attached to its N-terminus and is purified using specialized chromatographic methods.

Physical Appearance

A clear, sterile-filtered solution.

Formulation

The LRRC59 solution is provided at a concentration of 0.25 mg/ml and is prepared in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 2 mM DTT, 2 mM EDTA, and 50% glycerol.

Stability

For short-term storage (2-4 weeks), the solution can be kept at 4°C. For longer periods, store frozen at -20°C. It's recommended to add a carrier protein (0.1% HSA or BSA) for long-term storage. Avoid repeatedly freezing and thawing the solution.

Purity

Purity exceeds 90% as determined by SDS-PAGE analysis.

Synonyms

Leucine Rich Repeat Containing 59, Ribosome-Binding Protein P34, p34, Leucine-Rich Repeat-Containing Protein 59.

Source

Escherichia Coli.

Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MGSMTKAGSK GGNLRDKLDG NELDLSLSDL NEVPVKELAA LPKATILDLS CNKLTTLPSD FCGLTHLVKL DLSKNKLQQL PADFGRLVNL QHLDLLNNKL VTLPVSFAQL KNLKWLDLKD NPLDPVLAKV AGDCLDEKQC KQCANKVLQH MKAVQADQER ERQRRLEVER EAEKKREAKQ RAKEAQEREL RKREKAEEKE RRRKEYDALK AAKREQEKKP KKEANQAPKS KSGSRPRKPP PRKHTRS

Product Science Overview

Gene and Protein Structure

The LRRC59 gene is located on chromosome 17 and encodes a protein that is approximately 30.3 kDa in size. The protein consists of 267 amino acids, with a 23 amino acid His-tag at the N-terminus, which is used for purification purposes. The LRRC59 protein is a type II transmembrane protein, meaning it spans the membrane with its N-terminus inside the cell and its C-terminus outside.

Function and Localization

LRRC59 is involved in several cellular processes, including the regulation of nuclear import of fibroblast growth factor 1 (FGF1). It facilitates the interaction of FGF1 with the nuclear import machinery, aiding in its transport through nuclear pores. Additionally, LRRC59 has been predicted to play a role in positive regulation of Ras protein signal transduction and general signal transduction processes.

The protein is primarily localized in the endoplasmic reticulum and mitochondrial nucleoid. It exhibits cytoplasmic expression of varying intensity across most tissues.

Clinical Significance

Mutations or dysregulation of the LRRC59 gene have been associated with certain diseases, including Ogden Syndrome. Moreover, LRRC59 has been implicated in the development of various human malignancies, highlighting its potential role in cancer biology.

Recombinant Production

Recombinant LRRC59 is produced in E. coli as a single, non-glycosylated polypeptide chain. This recombinant form is used in various research applications to study the protein’s function and interactions.

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LRRC59 Human

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Product Specs

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