LRIG1 Mouse
Sf9, Baculovirus cells.
Leucine-rich repeats and immunoglobulin-like domains protein 1, LIG-1, Lrig1, LIG1, D6Bwg0781e, Img
Greater than 90.0% as determined by SDS-PAGE.
Description
LRIG1 Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 770 amino acids (35-796 a.a.) and having a molecular mass of 84.8kDa.
LRIG1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
The protein LRIG1, short for Leucine Rich Repeats And Immunoglobulin Like Domains 1, plays a crucial role in regulating the EGFR signaling pathway. LRIG1 acts as a general inhibitor of this pathway. Research indicates that LRIG1 interacts with ErbB proteins and Met kinase, influencing their regulation through mechanisms like increased ubiquitination and degradation within lysosomes. This makes LRIG1 a tumor suppressor, as it controls cell proliferation by negatively regulating tyrosine kinase receptors in the EGF protein family. Notably, LRIG1 expression is often reduced in cancerous cells and serves as a potential indicator for cancer progression.
Produced in Sf9 Baculovirus cells, LRIG1 Mouse is a single, glycosylated polypeptide chain consisting of 770 amino acids (specifically, amino acids 35 to 796). It has a molecular weight of 84.8kDa. The protein is engineered with an 8 amino acid His tag at its C-terminus and undergoes purification using proprietary chromatographic techniques.
LRIG1 protein solution is provided at a concentration of 0.5mg/ml. It is prepared in a buffer containing 10% glycerol and Phosphate Buffered Saline (pH 7.4).
For short-term storage (up to 2-4 weeks), the solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein like HSA or BSA (0.1%) is advisable for long-term storage to maintain protein stability. It is crucial to avoid repeated freeze-thaw cycles to prevent protein degradation.
Analysis by SDS-PAGE confirms that the purity of the LRIG1 Mouse protein is greater than 90%.
Leucine-rich repeats and immunoglobulin-like domains protein 1, LIG-1, Lrig1, LIG1, D6Bwg0781e, Img
Sf9, Baculovirus cells.
AQAGPRAPCA AACTCAGDSL DCSGRGLATL PRDLPSWTRS LNLSYNRLSE IDSAAFEDLT NLQEVYLNSN ELTAIPSLGA ASIGVVSLFL QHNKILSVDG SQLKSYLSLE VLDLSSNNIT EIRSSCFPNG LRIRELNLAS NRISILESGA FDGLSRSLLT LRLSKNRITQ LPVKAFKLPR LTQLDLNRNR IRLIEGLTFQ GLDSLEVLRL QRNNISRLTD GAFWGLSKMH VLHLEYNSLV EVNSGSLYGL TALHQLHLSN NSISRIQRDG WSFCQKLHEL ILSFNNLTRL DEESLAELSS LSILRLSHNA ISHIAEGAFK GLKSLRVLDL DHNEISGTIE DTSGAFTGLD NLSKLTLFGN KIKSVAKRAF SGLESLEHLN LGENAIRSVQ FDAFAKMKNL KELYISSESF LCDCQLKWLP PWLMGRMLQA FVTATCAHPE SLKGQSIFSV LPDSFVCDDF PKPQIITQPE TTMAVVGKDI RFTCSAASSS SSPMTFAWKK DNEVLANADM ENFAHVRAQD GEVMEYTTIL HLRHVTFGHE GRYQCIITNH FGSTYSHKAR LTVNVLPSFT KIPHDIAIRT GTTARLECAA TGHPNPQIAW QKDGGTDFPA ARERRMHVMP DDDVFFITDV KIDDMGVYSC TAQNSAGSVS ANATLTVLET PSLAVPLEDR VVTVGETVAF QCKATGSPTP RITWLKGGRP LSLTERHHFT PGNQLLVVQN VMIDDAGRYT CEMSNPLGTE RAHSQLSILP TPGCRKDGTT VGVEHHHHHH
Product Science Overview
The LRIG1 gene is a protein-coding gene that plays a significant role in various biological processes. The protein encoded by this gene contains multiple leucine-rich repeats and immunoglobulin-like domains, which contribute to its function in cellular signaling and interaction . The LRIG1 protein is a type-I transmembrane protein with 1092 amino acids and a molecular weight of approximately 120 kDa .
LRIG1 acts as a feedback negative regulator of signaling by receptor tyrosine kinases. It enhances receptor ubiquitination and accelerates intracellular degradation, thereby modulating signaling pathways that are crucial for cell growth and differentiation . This regulatory function is essential for maintaining cellular homeostasis and preventing uncontrolled cell proliferation.
LRIG1 is ubiquitously expressed in various tissues, including the skin, nervous system, and gastrointestinal tract . It is predominantly located in the plasma membrane but can also be found in the extracellular matrix and extracellular space . The expression of LRIG1 is tightly regulated and can be induced in response to specific cellular signals.
LRIG1 has been identified as a tumor suppressor gene in various types of cancer. It is highly expressed in tumor-infiltrating lymphocytes (TILs) and may play a role in modulating T cell activation . The expression of LRIG1 in TILs suggests that it could be involved in regulating the anti-tumor immune response, making it a potential target for cancer immunotherapy .
The study of LRIG1 has significant implications for understanding cancer biology and developing therapeutic strategies. By elucidating the mechanisms by which LRIG1 regulates receptor tyrosine kinase signaling and immune responses, researchers can identify new targets for cancer treatment and improve existing therapies .
