LPCAT1 Human

Lysophosphatidylcholine Acyltransferase Human Recombinant
Cat. No.
BT9680
Source
Escherichia Coli.
Synonyms
AYTL2, lpcat, PFAAP3, Lysophosphatidylcholine acyltransferase 1, LPC acyltransferase 1, LPCAT-1, LysoPC acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

LPCAT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 479 amino acids (79-534a.a) and having a molecular mass of 53.4kDa. LPCAT1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Lysophosphatidylcholine acyltransferase 1 (LPCAT1) is an enzyme that plays a crucial role in the remodeling of phospholipids, particularly phosphatidylcholine. It belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. LPCAT1 exhibits both acyltransferase and acetyltransferase activities and facilitates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) to phosphatidylcholine (PC). The enzyme demonstrates a preference for saturated fatty acyl-CoAs and utilizes 1-myristoyl or 1-palmitoyl LPC as acyl donors and acceptors, respectively. LPCAT1 is involved in the synthesis of phosphatidylcholine in pulmonary surfactant, highlighting its significance in respiratory function.
Description
Recombinant human LPCAT1, expressed in E. coli, is a monomeric, non-glycosylated polypeptide chain comprising 479 amino acids (residues 79-534). It has a molecular weight of 53.4 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The LPCAT1 solution is provided at a concentration of 0.25 mg/ml in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freeze-thaw cycles should be avoided.
Purity
The purity of the LPCAT1 protein is greater than 90%, as determined by SDS-PAGE analysis.
Synonyms
AYTL2, lpcat, PFAAP3, Lysophosphatidylcholine acyltransferase 1, LPC acyltransferase 1, LPCAT-1, LysoPC acyltransferase 1, 1-acylglycerophosphocholine O-acyltransferase, 1-alkylglycerophosphocholine O-acetyltransferase, Acetyl-CoA:lyso-platelet-activating factor acetyltransferase, Acetyl-CoA:lyso-PAF acetyltransferase, Lyso-PAF acetyltransferase, LysoPAFAT, Acyltransferase-like 2, Phosphonoformate immuno-associated protein 3.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSAEKEPE QPPALWRKVV DFLLKAIMRT MWFAGGFHRV AVKGRQALPT EAAILTLAPH SSYFDAIPVT MTMSSIVMKA ESRDIPIWGT LIQYIRPVFV SRSDQDSRRK TVEEIKRRAQ SNGKWPQIMI FPEGTCTNRT CLITFKPGAF IPGAPVQPVV LRYPNKLDTI TWTWQGPGAL EILWLTLCQF HNQVEIEFLP VYSPSEEEKR NPALYASNVR RVMAEALGVS VTDYTFEDCQ LALAEGQLRL PADTCLLEFA RLVRGLGLKP EKLEKDLDRY SERARMKGGE KIGIAEFAAS LEVPVSDLLE DMFSLFDESG SGEVDLRECV VALSVVCRPA RTLDTIQLAF KMYGAQEDGS VGEGDLSCIL KTALGVAELT VTDLFRAIDQ EEKGKITFAD FHRFAEMYPA FAEEYLYPDQ THFESCAETS PAPIPNGFCA DFSPENSDAG RKPVRKKLD.

Product Science Overview

Introduction

Lysophosphatidylcholine acyltransferase (LPCAT) is an enzyme that plays a crucial role in the remodeling of phospholipids, specifically in the conversion of lysophosphatidylcholine (LPC) to phosphatidylcholine (PC). This process is essential for maintaining the structure and functionality of cellular membranes. LPCAT is part of the 1-acyl-sn-glycerol-3-phosphate acyltransferase family and is evolutionarily conserved across various species .

Types of LPCAT

There are several isoforms of LPCAT, including LPCAT1, LPCAT2, LPCAT3, and LPCAT4. Each isoform has distinct functions and tissue distributions:

  • LPCAT1: Primarily involved in lung surfactant production and is crucial for lung function .
  • LPCAT2: Plays a role in inflammatory responses and is found in immune cells.
  • LPCAT3: Involved in lipid metabolism and is expressed in the liver and intestines .
  • LPCAT4: Functions in various tissues but its specific roles are less well understood.
Recombinant Human LPCAT

Recombinant human LPCAT refers to the enzyme produced through recombinant DNA technology, which allows for the production of large quantities of the enzyme for research and therapeutic purposes. This technology involves inserting the gene encoding LPCAT into a suitable expression system, such as bacteria or yeast, to produce the enzyme in vitro.

Functions and Mechanisms

LPCAT catalyzes the acylation of LPC to form PC, a major component of cellular membranes. This reaction is part of the Lands cycle, a phospholipid remodeling pathway that maintains the proper composition and fluidity of membranes . The enzyme’s activity is influenced by various factors, including pH, temperature, and the availability of substrates such as acyl-CoA .

Clinical Significance

LPCAT enzymes have been implicated in several diseases:

  • Atherosclerosis: LPCAT3 is involved in lipid metabolism and has been linked to the development of atherosclerosis .
  • Non-alcoholic Steatohepatitis (NASH): LPCAT3 plays a role in liver lipid metabolism and is associated with NASH .
  • Cancer: LPCAT1 has been identified as a potential biomarker for prognosis and immunotherapy response in various cancers .
Research and Applications

Research on recombinant human LPCAT has led to the development of assays to measure its activity and identify potential inhibitors and activators. For example, a novel assay based on reversed-phase HPLC has been developed to measure LPCAT3 activity, which may facilitate the identification of therapeutic targets for diseases such as atherosclerosis and NASH .

In conclusion, lysophosphatidylcholine acyltransferase is a vital enzyme in phospholipid metabolism with significant implications for human health. Recombinant technology has enabled detailed studies of its functions and potential therapeutic applications.

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