LMX1B Human
LIM Homeobox Transcription Factor 1, Beta Human Recombinant
Cat. No.
BT21073
Source
Escherichia Coli.
Synonyms
LIM homeobox transcription factor 1, beta, LMX1.2, NPS1, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX1B.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
LMX1B Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 418 amino acids (1-395a.a) and having a molecular mass of 46.5kDa.
LMX1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
LMX1B is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
LIM Homeobox Transcription Factor 1, Beta (LMX1B) belongs to the LIM-homeodomain protein family. These proteins are characterized by two N-terminal zinc-binding LIM domains, a homeodomain, and a C-terminal glutamine-rich domain. Functioning as a transcription factor, LMX1B is crucial for determining dorsal limb fate in the zeugopodal and autopodal stages of development. Mutations in the LMX1B gene have been linked to nailpatella syndrome.
Description
Recombinant human LMX1B protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It comprises 418 amino acids (1-395a.a), including a 23 amino acid His-tag at the N-terminus, and has a molecular weight of 46.5kDa. The protein is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless, and sterile-filtered solution.
Formulation
The LMX1B protein is supplied in a solution at a concentration of 1mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (up to 4 weeks), the protein can be stored at 4°C. For extended periods, it is recommended to store the protein frozen at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Repeated freezing and thawing of the protein should be avoided.
Purity
The purity of the LMX1B protein is greater than 80%, as determined by SDS-PAGE analysis.
Synonyms
LIM homeobox transcription factor 1, beta, LMX1.2, NPS1, LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX1B.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDIATGP ESLERCFPRG QTDCAKMLDG IKMEEHALRP GPATLGVLLG SDCPHPAVCE GCQRPISDRF LMRVNESSWH EECLQCAACQ QALTTSCYFR DRKLYCKQDY QQLFAAKCSG CMEKIAPTEF VMRALECVYH LGCFCCCVCE RQLRKGDEFV LKEGQLLCKG DYEKEKDLLS SVSPDESDSV KSEDEDGDMK PAKGQGSQSK GSGDDGKDPR RPKRPRTILT TQQRRAFKAS FEVSSKPCRK VRETLAAETG LSVRVVQVWF QNQRAKMKKL ARRHQQQQEQ QNSQRLGQEV LSSRMEGMMA SYTPLAPPQQ QIVAMEQSPY GSSDPFQQGL TPPQMPGNDS IFHDIDSDTS LTSLSDCFLG SSDVGSLQAR VGNPIDRLYS MQSSYFAS.
Product Science Overview
Introduction
LIM Homeobox Transcription Factor 1, Beta (LMX1B) is a protein encoded by the LMX1B gene in humans. This transcription factor is a member of the LIM-homeodomain family of proteins, which are characterized by the presence of two N-terminal zinc-binding LIM domains, a homeodomain, and a C-terminal glutamine-rich domain .
Gene and Protein Structure
The LMX1B gene is located on chromosome 9 and encodes a protein consisting of 402 amino acids . The protein structure includes:
- Two N-terminal LIM domains: These domains are involved in protein-protein interactions and are crucial for the transcriptional activity of LMX1B.
- Homeodomain: This domain is responsible for DNA binding and is essential for the transcriptional regulation of target genes.
- C-terminal glutamine-rich domain: This domain is involved in transcriptional activation .
Biological Functions
LMX1B plays a critical role in various developmental processes, including:
- Dorsal limb development: LMX1B is essential for the specification of dorsal limb structures at both the zeugopodal (forearm and lower leg) and autopodal (hand and foot) levels .
- Renal glomerular filtration barrier: It is involved in the regulation of podocyte-expressed genes, which are crucial for the integrity of the glomerular basement membrane .
- Anterior segment of the eye: LMX1B is necessary for the normal development of the anterior segment of the eye .
- Neuronal differentiation: It plays a role in the differentiation of dopaminergic and serotonergic neurons .
Clinical Significance
Mutations in the LMX1B gene are associated with several disorders, including:
Recombinant LMX1B
Human recombinant LMX1B is produced using recombinant DNA technology, which involves inserting the LMX1B gene into a suitable expression system, such as bacteria or mammalian cells. This allows for the production of large quantities of the protein for research and therapeutic purposes.
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