LIMD2 Human
LIM Domain Containing 2 Human Recombinant
Cat. No.
BT20496
Source
Escherichia Coli.
Synonyms
LIM Domain-Containing Protein 2
Appearance
Sterile filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
LIMD2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 150 amino acids (1-127) and having a molecular mass of 16.5kDa.
LIMD2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
LIMD2, a protein-coding gene, encodes a protein with one LIM zinc-binding domain. This gene is associated with diseases like intrahepatic cholangiocarcinoma and estrogen-receptor negative breast cancer.
Description
Recombinant human LIMD2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 150 amino acids (residues 1-127) and has a molecular weight of 16.5 kDa. This protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The LIMD2 solution is supplied in 20mM Tris-HCl buffer with a pH of 8.0, containing 0.15M NaCl, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer periods, store frozen at -20°C. Adding a carrier protein like 0.1% HSA or BSA is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% by SDS-PAGE analysis.
Synonyms
LIM Domain-Containing Protein 2
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMFQAAGA AQATPSHDAK GGGSSTVQRS KSFSLRAQVK ETCAACQKTV YPMERLVADK LIFHNSCFCC KHCHTKLSLG SYAALHGEFY CKPHFQQLFK SKGNYDEGFG RKQHKELWAH KEVDPGTKTA
Product Science Overview
Introduction
Structure and Function
Expression and Localization
Recombinant LIMD2
Recombinant LIMD2 refers to the artificially synthesized version of the protein, produced using recombinant DNA technology. This involves inserting the gene encoding LIMD2 into an expression system, such as bacteria or insect cells, to produce the protein in large quantities . The recombinant protein is often tagged with a marker, such as His6, to facilitate purification and characterization .
Applications
Recombinant LIMD2 is used in various research applications, including:
- Studying protein-protein interactions: Understanding how LIMD2 interacts with other proteins in signaling pathways.
- Investigating post-translational modifications: Analyzing how modifications affect the protein’s function and behavior.
- Developing therapeutic strategies: Exploring potential roles of LIMD2 in diseases and developing targeted therapies.
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